Protocols



Plate/slide selection



Fluorophore selection

Below are the most popular fluorescent dyes and their compatibility with the facility's microscopes


   Compatibility
Fluorophore Celígo IN Cell 6000
  DAPI Perfect Perfect
Hoechst Perfect Perfect
AlexaFluor® 488 Perfect Perfect
GFP Perfect Perfect
Calcein Perfect Perfect
FITC (if possible, should be replaced with AlexaFluor® 488) Perfect Perfect
AlexaFluor® 514 Good (not in combination with red dye) Good (not in combination with red dye)
YFP Good (not in combination with red dye) Good (not in combination with red dye)
AlexaFluor® 532 Fair (not in combination with red dye) Fair (not in combination with red dye)
Propidium Iodide (PI) Perfect Perfect
AlexaFluor® 546 Good Perfect
AlexaFluor® 555 Good Perfect
AlexaFluor® 568 Good Perfect
AlexaFluor® 594 Fair Perfect
Texas Red Fair Perfect
DRAQ5 N/A Perfect
Cy5 N/A Perfect
AlexaFluor® 633 N/A Perfect
AlexaFluor® 647 N/A Perfect


If fluorophore of interest is missing, please contact the facility or use Fluorescence SpectraViewer, using the following excitation/emission information:


Channel Celígo IN Cell 6000
Excitation Dichroic Emission Laser Emission
Blue 377 / 50 409 470 / 22 405 455 / 50
Green 483 / 32 506 536 / 40 488 525 / 20
Red 531 / 40 593 629 / 53 561 605 / 52
Far-red N/A 640 706.5 / 72


Sample preparation



Live imaging