About Us






The Network Biology Collaborative Centre (NBCC) is located at the Lunenfeld-Tanenbaum Research Institute (LTRI) in Mount Sinai Hospital. The Centre was formed in 2014 from the amalgamation of two flagship LTRI facilities: The Proteomics Facility for mass spectrometry-based analysis of protein function and the SMART High-Throughput and High-Content Screening laboratory for functional genomics studies. The Centre is a member of the Genome Canada Genomics Innovation Network.


The mandate of the Centre is to assist Canadian and International scientists in their investigation of complex biological networks related to human health and disease by providing access to advanced instrumentation, proven workflows, and world-class expertise in functional proteomics and genomics.


Our Beliefs:

We believe that the best approach to understanding the complexities of biological networks is to integrate functional genomics approaches designed to identify genes of interest with proteomics analysis to demonstrate the function of the encoded proteins.

We have devoted considerable resources into building the infrastructure, methodology, and integrative data analysis pipelines to effectively study biological networks, and we will continue to do so as we believe that innovating, improving, and implementing new technology is the best way to maintain the highest-calibre research. And we believe that all research at the NBCC should be performed at this level.


Our Users:

As a national platform, we work with users from across Canada and Internationally. We accept both small and large-scale projects. We encourage any scientist with a project that could fit our expertise to contact us.


Our Funders:

The Centre is supported by funding from the Canadian Foundation for Innovation (CFI), The Ontario Ministry of Research and Innovation, Genome Canada through Ontario Genomics, Canadian Institutes of Health Research (CIHR), and the Lunenfeld-Tanenbaum Research Institute.



Jeff Wrana

Co-Director of the Network Biology Collaborative Centre


Dr. Jeff Wrana is a Senior Investigator at the Lunenfeld-Tanenbaum Research Institute and Professor in the Department of Molecular Genetics, University of Toronto. He is internationally recognized for his work on TGFb signal transduction in development and cancer. He has won numerous awards for his work, including the Gertrude B. Elion prize from the AACR, the William E. Rawls Prize from the NCIC (now CCSRI), the Paul Marks prize from Sloan Kettering (NY) and the Diamond Jubilee Medal from the Prime Minister of Canada. Dr. Wrana founded the LTRI high-throughput biology and high-content imaging centres and has been instrumental in promoting the widespread application of systematic screens using robotics to address biological problems.

Wrana lab website





Anne-Claude Gingras

Co-Director of the Network Biology Collaborative Centre


Dr. Anne-Claude Gingras is a Senior Investigator at the Lunenfeld-Tanenbaum and Associate Professor in the Department of Molecular Genetics, University of Toronto. She holds the Canada Research Chair in Functional Proteomics and the Lea Reichmann Chair in Cancer Proteomics. In 2011, she was recognized as one of Canada's top 100 most powerful women. Her core biological interests centre on signal transduction in cancer and vascular diseases. Dr. Gingras is Director of Proteomics and has focused on applying mass spectrometry-based proteomics to understanding the assembly of proteins into complex signaling networks.

Gingras lab website





Laurence Pelletier

Principal Investigator, High Spatio-temporal Resolution Imaging


Dr. Laurence Pelletier is a Senior Investigator at the Lunenfeld-Tanenbaum Research Institute, and an Associate Professor in the Department of Molecular Genetics at the University of Toronto. Dr. Pelletier is an expert in functional genomics, high-resolution microscopy and image analysis applied to the study of mitotic processes, organelle biogenesis (in particular centrosomes and cilia) and chromosome segregation. Dr. Pelletier holds a Canada Research Chair in Centrosome Biogenesis and Function and was awarded the 2009 Early Researcher Award from the Ontario Ministry of Research and Innovation. Over the years, Dr. Pelletier has established a fruitful relationship with GE healthcare, which will be instrumental in the tailoring and further development of the imaging methodologies at the LTRI.

Pelletier lab website





Karen Colwill

Network Biology Collaborative Centre Manager


Dr. Karen Colwill is a Staff Scientist at the Lunenfeld-Tanenbaum Research Institute in addition to being the Manager of the Network Biology Collaborative Centre. She has over 15 years experience in managing scientific projects and facilities. As the Centre's Manager, Karen is responsible for daily oversight of the centre, coordinating external customer requests between the divisions, and liaising with the individual division managers and directors.





Brett Larsen

Proteomics Manager


Brett Larsen has over 15 years experience in mass spectrometry and he has been managing the Proteomics division since 2004. He is an expert in quantitative proteomics, post-translational modifications and data analysis. He is responsible for equipment oversight, technology development, and user training. As the manager, Brett works with users from project inception to project completion.

Proteomics




Alessandro Datti

High-Throughput Screening Manager


Dr. Alessandro Datti supervises and coordinates the scientific operations of the High-Throughput Screening division, and serves as a senior Lecturer of Biochemistry at the University of Perugia (Italy). His main research expertise and competencies are dedicated to the strategic design, development and validation of biological/biochemical assays for applications in high-throughput, robot-assisted formats aimed at identifying molecular entities with biological or pharmacological properties.

High-Throughput Screening





Mikhail (Misha) Bashkurov

High-Content Screening Manager


Dr. Mikhail Bashkurov has been the manager of the high-content screening division since 2012. He has over 10 years experience in microscopy as well as image analysis. As the manager, Mikhail assists the users at every step of the screening process from screen design to data analysis and statistics. He is also involved in instrument maintenance and training of new users.

High-Content Screening





Monica Hasegan

High Spatio-temporal Resolution Imaging Manager


Dr. Monica Hasegan is in charge of the high-resolution and super-resolution optical microscopes within the NBCC. With a PhD in Physics, she has a strong background in instrumentation, advanced imaging, data processing and analysis (including denoising algorithms and automation of high-throughput applications), high performance computing on cluster of computers and data management. She assists users with microscopy training, as well as standard and custom-made image analyses.

High Spatio-Temporal Resolution Imaging





Kin Chan

Sequencing Manager


Kin Chan (MSc) has worked on high-throughput data screening platforms for more than fifteen years and has managed both microarray and sequencing facilities. He has in-depth technical and scientific knowledge of genomics with a focus on transcriptomics profiling. His current focus is to translate scientific finding to clinical use by working with research investigators, physicians and clinical labs. Kin was instrumental in our sequencing division being successfully accredited by ISO 15189.





Miriam Barrios-Rodilles

Scientific Associate, LUMIER Specialist


Expertise: mammalian network proteomics by LUMIER, functional high-throughput (HT) screens, cell-based HT-assay development

Email: miriam@lunenfeld.ca
 




James Knight

Research Associate in Proteomics Data Analysis and Visualization


Expertise: data visualization, data processing, proteomics sample preparation

Email: jknight@lunenfeld.ca
 




Zhen-Yuan Lin

Biology and Mass Spectrometry Research Technician


Expertise: sample purification and mass spectrometry operation

Email: zlin@lunenfeld.ca
 




Guomin (Frank) Liu

Programmer


Expertise: ProHits LIMS system, software integration

Email: gliu@lunenfeld.ca
 




Mark Jen

Laboratory Automation Technician


Expertise: routine maintenance and QC of instrumentation, development and implementation of robot-assisted technologies

Email: mjen@lunenfeld.ca 




Adrian Pasculescu

Software Engineer


Expertise: data analysis and integration

Email: pasculescu@lunenfeld.ca
 




Jess Shen

Bioinformatician


Expertise: next-generation sequencing and protein-protein interaction network analysis

Email: shen@lunenfeld.ca
 




Johnny Tkach

Research Associate


Expertise: CRISPR-mediated genome editing, chemical-genetic screening, high-throughput fluorescence microscopy and image analysis

Email: tkach@lunenfeld.ca
 




Jenny Wang

Lab Automation Specialist


Expertise: development, optimization and execution of robotic methods in high-throughput formats, routine maintenance of robotic equipment

Email:jwang@lunenfeld.ca
 




JianPing Zhang

Programmer


Expertise: ProHits LIMS system, user interfaces

Email: jpzhang@lunenfeld.ca