Resources

Preparation

The best quality results derive from the best experimental setup. To ensure that the samples we receive are of the highest quality, we provide our users and the scientific community with user guides for each facility, protocols for sample preparation and analysis, and reagents that will assist them with performing the optimal sample preparation prior to analysis. We also host seminars, workshops and provide in-facility training to inform users on how they may apply our services to their biological question. If you have questions related to our services that are not answered in this resource, please contact the applicable Facility Manager.

Sequencing Robot

Guides

Facility Guide
Proteomics
Next-Generation Sequencing
High-Throughput Screening
High-Content Screening
Advanced Imaging
Flow Cytometry

Protocols

Detailed protocols can be found on the individual facility pages.

Reagents

The NBCC is pleased to provide a number of vectors for use. To request a vector, please email clonerequest@lunenfeld.ca.

These vectors are being provided in collaboration with the Gingras lab to assist scientists in their functional proteomics and COVID-19 neutralization experiments. We ask that you acknowledge their use in your publications by citing the appropriate reference.

Proximity-dependent biotinylation stable transfection vectors
Name Description PDF GBK
N-miniTurbo N-term miniTurbo-3xFLAG with gateway cloning site
C-miniTurbo C-term miniTurbo-3xFLAG with gateway cloning site

Rosenthal, S.M., T. Misra, H. Abdouni, T.C. Branon, A.Y. Ting, I.C. Scott, A.C. Gingras, A toolbox for efficient proximity-dependent biotinylation in zebrafish embryos. Mol Cell Proteomics, 2021. 20:100128.

Proximity-dependent biotinylation lentiviral vectors
Name Description PDF GBK
N-pSTV2 miniTurbo N-term miniTurbo-3xFLAG with gateway cloning site; pTight promoter
C-pSTV2 miniTurbo C-term miniTurbo-3xFLAG with gateway cloning site; pTight promoter
N-pSTV6 miniTurbo N-term miniTurbo-3xFLAG with gateway cloning site; pTight promoter; Puro-2A-rtTA driven by PGK promoter
C-pSTV6 miniTurbo C-term miniTurbo-3xFLAG with gateway cloning site; pTight promoter; Puro-2A-rtTA driven by PGK promoter
N-pSTV2 N-term BirA*-Flag with gateway cloning site; pTight promoter
C-pSTV2 C-term BirA*-Flag with gateway cloning site; pTight promoter
N-pSTVH5 N-term BirA*-Flag with gateway cloning site; pTight promoter; YFP-2a-rtTA driven by sEF1a promoter
C-pSTVH5 C-term BirA*-Flag with gateway cloning site; pTight promoter; YFP-2a-rtTA driven by sEF1a promoter
N-pSTV6 N-term BirA*-Flag with gateway cloning site; pTight promoter; Puro-2A-rtTA driven by PGK promoter
C-pSTV6 C-term BirA*-Flag with gateway cloning site; pTight promoter; Puro-2A-rtTA driven by PGK promoter
N-pSTVH7 N-term BirA*-Flag with gateway cloning site; pTight promoter; Puromycin resistance gene driven by sEF1a promoter
C-pSTVH7 C-term BirA*-Flag with gateway cloning site; pTight promoter; Puromycin resistance gene driven by sEF1a promoter
N-pSTVH8 N-term BirA*-Flag with gateway cloning site; pTight promoter; rtTA driven by sEF1a promoter
C-pSTVH8 C-term BirA*-Flag with gateway cloning site; pTight promoter; rtTA driven by sEF1a promoter

Samavarchi-Tehrani, P., H. Abdouni, R. Samson, and A.C. Gingras, A versatile lentiviral delivery toolkit for proximity-dependent biotinylation in diverse cell types. Mol Cell Proteomics, 2018. 17(11):2256-2269.


Samavarchi-Tehrani, P., H. Abdouni, J.D.R. Knight, A. Astori, R. Samson, Z-Y. Lin, D.-K. Kim, J.J. Knapp, J. St-Germain, C.D. Go, B. Larsen, C.J. Wong, P. Cassonnet, C. Demeret, Y. Jacob, F.P. Roth, B. Raught, A.C. Gingras, A SARS-CoV-2 – host proximity interactome. bioRxiv, 2020.

Pseudotyped-Spike Lentiviral Vectors
Name Description PDF GBK
pTwist Amp B.1.1.7 Spike B.1.1.7 Spike cDNA, not an expression plasmid
pTwist Amp B.1.351 Spike B.1.351 Spike cDNA, not an expression plasmid
pTwist Amp B.1.617.2 Spike B.1.617.2 Spike cDNA, not an expression plasmid
pTwist-Amp P.1 Spike P.1 Spike cDNA, not an expression plasmid
pTwist Amp Wuhan-1 Spike Wuhan 1 Spike cDNA, not an expression plasmid
HDM Omicron (B.1.1529) Spike Omicron Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM BA.2 Spike BA.2 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM BA.2.12.1 Spike BA.2.12.1 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM BA.2.75 Spike BA.2.75 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM BA.2.75.2 Spike BA.2.75.2 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM BA.5 Spike BA.5 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM BF.7 Spike BF.7 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM BQ.1 Spike BQ.1 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM BQ.1.1 Spike BQ.1.1 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM XBB.1 Spike XBB.1 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM XBB.1.5 Spike XBB.1.5 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM XBB.1.16 Spike XBB.1.16 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM EG.5.1 Spike EG.5.1 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM JN.1 Spike JN.1 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM KP.2 Spike KP.2 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM B.1.1.318 Spike B.1.1.318 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM B.1.1.7 Spike B.1.1.7 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM B.1.351 Spike B.1.351 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM B.1.617.2 Spike B.1.617.2 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM B.1.621 Spike B.1.621 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM Delta+ (B.1.617.2 K417N) Spike Delta+ (B.1.617.2_K417N) Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM Delta Q613H Spike Delta Q613H Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM Delta Y145H Spike Delta Y145H Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM Lambda Spike Lambda Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM P.1 Spike P.1 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM Wuhan-1 D614G Wuhan-1 D614G cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells
HDM Wuhan-1 Spike Wuhan-1 Spike cDNA with CMV enhancer/promoter for cDNA expression in mammalian cells

The HDM reagents were generated by Rod Hardy for CoVaRR-Net. Please cite CoVaRR-Net and link to the website covarrnet in your publications.

Last updated: September 24, 2024

NBCC

Network Biology Collaborative Centre

Sinai Health, Lunenfeld-Tanenbaum Research Institute
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