Plasmid Vectors



The NBCC is pleased to provide the following lentiviral vectors for use:

Plasmid ID
Plasmid Name
Plasmid Description
Plasmid Map
Plasmid Sequence
pSTV2 N-term
N-term BirA*-Flag with gateway cloning site; pTight promoter
pSTV2 C-term
C-term BirA*-Flag with gateway cloning site; pTight promoter
pSTVH5 N-term
N-term BirA*-Flag with gateway cloning site; pTight promoter; YFP-2a-rtTA driven by sEF1a promoter
pSTVH5 C-term
C-term BirA*-Flag with gateway cloning site; pTight promoter; YFP-2a-rtTA driven by sEF1a promoter
pSTV6 N-term
N-term BirA*-Flag with gateway cloning site; pTight promoter; Puro-2A-rtTA driven by PGK promoter
pSTV6 C-term
C-term BirA*-Flag with gateway cloning site; pTight promoter; Puro-2A-rtTA driven by PGK promoter
pSTVH7 N-term
N-term BirA*-Flag with gateway cloning site; pTight promoter; Puromycin resistance gene driven by sEF1a promoter
pSTVH7 C-term
C-term BirA*-Flag with gateway cloning site; pTight promoter; Puromycin resistance gene driven by sEF1a promoter
pSTVH8 N-term
N-term BirA*-Flag with gateway cloning site; pTight promoter; rtTA driven by sEF1a promoter
pSTVH8 C-term
C-term BirA*-Flag with gateway cloning site; pTight promoter; rtTA driven by sEF1a promoter


To request a vector, please contact Hala Abdouni (habdouni @ lunenfeld.ca)


These vectors are being provided in collaboration with the Gingras lab to assist scientists in their functional proteomics experiments. We ask that you acknowledge their use in your publications by citing:


Samavarchi-Tehrani, P., H. Abdouni, R. Samson, and A.C. Gingras, A versatile lentiviral delivery toolkit for proximity-dependent biotinylation in diverse cell types. Mol Cell Proteomics, 2018. epub ahead of publication.