LOCUS       pDEST_pcDNA5_3xFLAG-GS2-miniTurboID_C-term      7692 bp    DNA   CIRCULAR        04-06-2024
DEFINITION  Gateway Parent Destination Vector, Gateway destination vector with C-terminal 3xFLAG-miniTurboID
ACCESSION   V12624
KEYWORDS    
SOURCE      
  ORGANISM  
REFERENCE   1 - 7692 (bases 1 to 7692)
  AUTHORS   Alice Ting / Anne-Claude
  JOURNAL   
COMMENT     This vector replaces the C-terminal tag from V11923 with synthesized 3xFLAG-GS2-miniTurboID. miniTurboID does not contain a start codon.
FEATURES             Location/Qualifiers
     source          1..7692
     misc_feature    0..0
                     /note=5' linker
                     /gene=
                     /SECDrawas=Region
     misc_feature    0..0
                     /note=3' linker
                     /gene=
                     /SECDrawas=Region
     rep_origin      complement(5937..6525)
                     /note=origin of replication
                     /gene=pBR322
                     /SECDrawas=Region
     polyA_signal    3650..3874)
                     /note=polya tail
                     /gene=BGH
                     /SECDrawas=Region
     polyA_signal    5365..5486)
                     /note=polya tail
                     /gene=SV40
                     /SECDrawas=Region
     CDS             2221..2526
                     /note=selectable marker
                     /gene=ccdB
                     /SECDrawas=Region
     CDS             complement(6696..7556)
                     /note=selectable marker
                     /gene=Ampicillin Resistance
                     /SECDrawas=Region
     CDS             4213..5235
                     /note=selectable marker
                     /gene=Hygromycin Resistance without ATG
                     /SECDrawas=Region
     CDS             1220..1879
                     /note=selectable marker
                     /gene=Chloramphenicol Resistance
                     /SECDrawas=Region
     misc_feature    complement(2567..2691)
                     /note=3' cloning site
                     /gene=attR2
                     /SECDrawas=Region
     misc_feature    complement(5535..5551)
                     /note=miscellaneous
                     /gene=M13-rev
                     /SECDrawas=Region
     misc_feature    2780..2815
                     /note=miscellaneous
                     /gene=GS linker
                     /SECDrawas=Region
     misc_feature    4158..4205
                     /note=miscellaneous
                     /gene=FRT site
                     /SECDrawas=Region
     CDS             2714..2779
                     /note=tag
                     /gene=Triple Flag
                     /SECDrawas=Region
     CDS             2816..3589
                     /note=tag
                     /gene=miniTurboID
                     /SECDrawas=Region
     CDS             0..0
                     /organism=
                     /gene=
                     /note=accession: 
                     /note=
                     /SECDrawas=Gene
     promoter        complement(5583..5613)
                     /note=promoter
                     /gene=LAC
                     /SECDrawas=Region
     promoter        235..614)
                     /note=promoter
                     /gene=CMV enhancer
                     /SECDrawas=Region
     promoter        615..818)
                     /note=promoter
                     /gene=CMV
                     /SECDrawas=Region
     promoter        820..859)
                     /note=promoter
                     /gene=tetO2
                     /SECDrawas=Region
     promoter        5559..5575)
                     /note=promoter
                     /gene=LacO
                     /SECDrawas=Region
     promoter        complement(7557..7661)
                     /note=promoter
                     /gene=Ampicillin
                     /SECDrawas=Region
     misc_feature    987..1111
                     /note=5' cloning site
                     /gene=attR1
                     /SECDrawas=Region

ORIGIN

        1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 
       61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 
      121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 
      181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 
      241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 
      301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 
      361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 
      421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 
      481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 
      541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 
      601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 
      661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 
      721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 
      781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CCCTATCAGT GATAGAGATC 
      841 TCCCTATCAG TGATAGAGAT CGTCGACGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA 
      901 GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA 
      961 CTCTAGCGTT TAAACTTAAG CTTTAGACAA GTTTGTACAA AAAAGCTGAA CGAGAAACGT 
     1021 AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT 
     1081 ACTGTAAAAC ACAACATATC CAGTCACTAT GGCGGCCGCA TTAGGCACCC CAGGCTTTAC 
     1141 ACTTTATGCT TCCGGCTCGT ATAATGTGTG GATTTTGAGT TAGGATCCGT CGAGATTTTC 
     1201 AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC 
     1261 CCAATGGCAT CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA 
     1321 CCAGACCGTT CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA 
     1381 GTTTTATCCG GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG 
     1441 TATGGCAATG AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT 
     1501 TTTCCATGAG CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG 
     1561 GCAGTTTCTA CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT 
     1621 CCCTAAAGGG TTTATTGAGA ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC 
     1681 CAGTTTTGAT TTAAACGTGG CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG 
     1741 CAAATATTAT ACGCAAGGCG ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC 
     1801 CGTTTGTGAT GGCTTCCATG TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA 
     1861 GTGGCAGGGC GGGGCGTAAA GATCTGGATC CGGCTTACTA AAAGCCAGAT AACAGTATGC 
     1921 GTATTTGCGC GCTGATTTTT GCGGTATAAG AATATATACT GATATGTATA CCCGAAGTAT 
     1981 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT 
     2041 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTCT GGTAAGCACA ACCATGCAGA 
     2101 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG 
     2161 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCTGA CGAGAACAGG GGCTGGTGAA 
     2221 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA 
     2281 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT 
     2341 CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC 
     2401 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG 
     2461 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA 
     2521 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGACCATA GTGACTGGAT 
     2581 ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT 
     2641 GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTCTTG TACAAAGTGG TTGATTCTAG 
     2701 AATCGATGGT ACCGACTACA AGGACCACGA CGGTGACTAC AAGGACCACG ACATCGACTA 
     2761 CAAGGACGAC GACGACAAGG GGGGAGGGGG AGGATCTGGG GGAGGGGGAG GATCTGCGAT 
     2821 CCCGCTGCTG AACGCTAAAC AGATTCTGGG ACAGCTGGAC GGCGGGAGCG TGGCAGTCCT 
     2881 GCCTGTGGTC GACTCCACCA ATCAGTACCT GCTGGATCGA ATCGGCGAGC TGAAGAGTGG 
     2941 GGATGCTTGC ATTGCAGAAT ATCAGCAGGC AGGGAGAGGA AGCAGAGGGA GGAAATGGTT 
     3001 CTCTCCTTTT GGAGCTAACC TGTACCTGAG TATGTTTTGG CGCCTGAAGC GGGGACCAGC 
     3061 AGCAATCGGC CTGGGCCCGG TCATCGGAAT TGTCATGGCA GAAGCGCTGC GAAAGCTGGG 
     3121 AGCAGACAAG GTGCGAGTCA AATGGCCCAA TGACCTGTAT CTGCAGGATA GAAAGCTGGC 
     3181 AGGCATCCTG GTGGAGCTGG CCGGAATAAC AGGCGATGCT GCACAGATCG TCATTGGCGC 
     3241 CGGGATTAAC GTGGCTATGA GGCGCGTGGA GGAAAGCGTG GTCAATCAGG GCTGGATCAC 
     3301 ACTGCAGGAA GCAGGGATTA ACCTGGACAG GAATACTCTG GCCGCTATGC TGATCCGAGA 
     3361 GCTGCGGGCA GCCCTGGAAC TGTTCGAGCA GGAAGGCCTG GCTCCATATC TGTCACGGTG 
     3421 GGAGAAGCTG GATAACTTCA TCAATAGACC CGTGAAGCTG ATCATTGGGG ACAAAGAGAT 
     3481 TTTCGGGATT AGCCGGGGGA TTGATAAACA GGGAGCCCTG CTGCTGGAAC AGGACGGAGT 
     3541 TATCAAACCC TGGATGGGCG GAGAAATCAG TCTGCGGTCT GCCGAAAAGT AAGGCCGGCC 
     3601 CCTAGGCTCG AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT 
     3661 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC 
     3721 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT 
     3781 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT 
     3841 AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG 
     3901 GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     3961 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     4021 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     4081 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     4141 GATGGTTCAC GTACCTAGAA GTTCCTATTC CGAAGTTCCT ATTCTCTAGA AAGTATAGGA 
     4201 ACTTCCTTGG CCAAAAAGCC TGAACTCACC GCGACGTCTG TCGAGAAGTT TCTGATCGAA 
     4261 AAGTTCGACA GCGTCTCCGA CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC 
     4321 AGCTTCGATG TAGGAGGGCG TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC 
     4381 TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT TCCGGAAGTG 
     4441 CTTGACATTG GGGATTTCAG CGAGAGCCTG ACCTATTGCA TCTCCCGCCG TGCACAGGGT 
     4501 GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG 
     4561 GCCATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA 
     4621 CCGCAAGGAA TCGGTCAATA CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC 
     4681 CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT 
     4741 CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG 
     4801 GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA TAACAGCGGT CATTGACTGG 
     4861 AGCGAGGCGA TGTTCGGGGA TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG 
     4921 TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA 
     4981 GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC 
     5041 TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC 
     5101 CGATCCGGAG CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG 
     5161 ACCGATGGCT GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG 
     5221 AGGGCAAAGG AATAGCACGT ACTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG 
     5281 TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC 
     5341 ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA 
     5401 AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT 
     5461 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC 
     5521 TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA 
     5581 CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA 
     5641 CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG 
     5701 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC 
     5761 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT 
     5821 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 
     5881 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC 
     5941 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 
     6001 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT 
     6061 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG 
     6121 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 
     6181 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT 
     6241 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 
     6301 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC 
     6361 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC 
     6421 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 
     6481 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC 
     6541 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 
     6601 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 
     6661 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 
     6721 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 
     6781 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC 
     6841 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 
     6901 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 
     6961 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC 
     7021 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 
     7081 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 
     7141 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 
     7201 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 
     7261 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 
     7321 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 
     7381 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 
     7441 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 
     7501 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 
     7561 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7621 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7681 CCACCTGACG TC  
//
