LOCUS       pSTV6-C-mTurbo_p       11366 bp    DNA     circular     21-JAN-2019
DEFINITION  Step2) PCR Fragment A.
ACCESSION   urn.local...63-a0otn9y
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com Cat.
            No. 632158,632162,632157,632163.
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label="5'\LTR"
     misc_binding    636..653
                     /label="PBS"
     misc_feature    685..822
                     /label="Packaging\signal"
     misc_binding    1303..1536
                     /label="RRE"
     misc_feature    2028..2151
                     /label="cPPT/CTS"
     CDS             complement(2472..3245)
                     /created_by="User"
                     /label="miniTurbo(humanized)"
     CDS             complement(3246..3281)
                     /created_by="User"
                     /label="GS linker"
     CDS             complement(3282..3347)
                     /created_by="User"
                     /label="3XFLAG"
     attR2           3370..3494
                     /created_by="User"
                     /modified_by="User"
                     /label="AttR2"
     CDS             complement(3535..3840)
                     /created_by="User"
                     /label="ccdB"
     CDS             complement(4182..4841)
                     /created_by="User"
                     /label="CmR"
     attR1           complement(4950..5074)
                     /created_by="User"
     promoter        complement(5104..5419)
                     /created_by="User"
                     /label="TRE"
     promoter        5491..6003
                     /ApEinfo_fwdcolor="#00ff0a"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="PGK promoter"
     gene            6012..6599
                     /ApEinfo_fwdcolor="#004de2"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Puro-variant"
     misc_feature    6600..6662
                     /ApEinfo_fwdcolor="#00e20a"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="2A"
     misc_feature    6663..7409
                     /ApEinfo_fwdcolor="#00abff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="rtTA"
     misc_feature    7446..7973
                     /ApEinfo_fwdcolor="#8c00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE"
     LTR             8082..8718
                     /label="3'\LTR"
     rep_origin      complement(9187..9860)
                     /label="pUC\origin"
     CDS             complement(10005..11001)
                     /label="Amp(R)"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtg catgcctgct
     2221 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     2281 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
     2341 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
     2401 agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctctagact cgagcctagg
     2461 ggccggcctt acttttcggc agaccgcaga ctgatttctc cgcccatcca gggtttgata
     2521 actccgtcct gttccagcag cagggctccc tgtttatcaa tcccccggct aatcccgaaa
     2581 atctctttgt ccccaatgat cagcttcacg ggtctattga tgaagttatc cagcttctcc
     2641 caccgtgaca gatatggagc caggccttcc tgctcgaaca gttccagggc tgcccgcagc
     2701 tctcggatca gcatagcggc cagagtattc ctgtccaggt taatccctgc ttcctgcagt
     2761 gtgatccagc cctgattgac cacgctttcc tccacgcgcc tcatagccac gttaatcccg
     2821 gcgccaatga cgatctgtgc agcatcgcct gttattccgg ccagctccac caggatgcct
     2881 gccagctttc tatcctgcag atacaggtca ttgggccatt tgactcgcac cttgtctgct
     2941 cccagctttc gcagcgcttc tgccatgaca attccgatga ccgggcccag gccgattgct
     3001 gctggtcccc gcttcaggcg ccaaaacata ctcaggtaca ggttagctcc aaaaggagag
     3061 aaccatttcc tccctctgct tcctctccct gcctgctgat attctgcaat gcaagcatcc
     3121 ccactcttca gctcgccgat tcgatccagc aggtactgat tggtggagtc gaccacaggc
     3181 aggactgcca cgctcccgcc gtccagctgt cccagaatct gtttagcgtt cagcagcggg
     3241 atcgcagatc ctccccctcc cccagatcct ccccctcccc ccttgtcgtc gtcgtccttg
     3301 tagtcgatgt cgtggtcctt gtagtcaccg tcgtggtcct tgtagtcggt accatcgatt
     3361 ctagaatcaa ccactttgta caagaaagct gaacgagaaa cgtaaaatga tataaatatc
     3421 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     3481 atccagtcac tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat
     3541 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc
     3601 cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca
     3661 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag
     3721 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg
     3781 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat
     3841 ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct
     3901 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat
     3961 tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg
     4021 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac
     4081 atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac
     4141 gcatactgtt atctggcttt tagtaagccg gatccagatc tttacgcccc gccctgccac
     4201 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     4261 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     4321 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     4381 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     4441 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     4501 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     4561 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     4621 cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     4681 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     4741 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     4801 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     4861 gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga agcataaagt
     4921 gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt
     4981 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     5041 acgtttctcg ttcagctttt ttgtacaaac ttgtctaaag cttaggccgc ggatccttct
     5101 ccaggcgatc tgacggttca ctaaacgagc tctgcttata taggcctccc accgtacacg
     5161 cctacctcga catacgttct ctatcactga tagggagtaa actcgacata cgttctctat
     5221 cactgatagg gataaactcg acatacgttc tctatcactg atagggagta aactcgacat
     5281 acgttctcta tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag
     5341 taaactcgac atcgttctct atcactgata gggagtaaac tcgacatacg ttctctatca
     5401 ctgataggga gtaaactcta gtgaagacga aagggccaga cgcggccgct caacgcgtcc
     5461 ggtccaccac caccaccacc actaaggatc cggggttggg gttgcgcctt ttccaaggca
     5521 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     5581 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     5641 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     5701 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     5761 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     5821 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     5881 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     5941 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     6001 agcaattcac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6061 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6121 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6181 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6241 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6301 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6361 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6421 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6481 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6541 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6601 ccggtgccga aggtagaggt tctctcctca cttgtggtga tgttgaagaa aaccctggtc
     6661 caatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     6721 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     6781 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     6841 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     6901 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     6961 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     7021 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     7081 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     7141 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     7201 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     7261 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     7321 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     7381 attttgacct tgacatgctc cccgggtaac taagtaagga tcgatccaag atatcgtatt
     7441 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7501 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7561 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7621 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7681 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7741 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     7801 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7861 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7921 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7981 ccgcctgttt cgcctcggcg tccggactag aggtaccttt aagaccaatg acttacaagg
     8041 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     8101 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     8161 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     8221 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     8281 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     8341 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     8401 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     8461 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     8521 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8581 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8641 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8701 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8761 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
     8821 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8881 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8941 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9001 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9061 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9661 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10141 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10321 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10621 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10981 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
    11041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    11161 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    11221 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    11281 aaattgtatt tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt
    11341 taaatatgta ctacaaactt agtagt
//
