LOCUS       Exported                8352 bp DNA     circular SYN 20-JUL-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="color: #------"
     CDS             1422..5234
                     /codon_start=1
                     /label=B.1.1.7_Spike_cDNA
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAISGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIF
                     GTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYHKNNKSWMESEFRVYSSANNC
                     TFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEP
                     LVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGT
                     ITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFN
                     ATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVI
                     RGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNL
                     KPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHA
                     PATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIDDTTDAVRDP
                     QTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYST
                     GSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSHRRARSVASQSIIAYTM
                     SLGAENSVAYSNNSIAIPINFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQY
                     GSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKR
                     SFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYT
                     SALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGK
                     IQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILARLDKVEAEVQ
                     IDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMS
                     FPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFY
                     EPQIITTHNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDI
                     SGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIV
                     MVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     polyA_signal    5332..5726
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6146..6250
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6251..7111
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6251 .. 6319 / #ccffcc / signal sequence
                        2: 6320 .. 7111 / #ccffcc
                     Cleavage site after base 6319"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7282..7870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattccagt gcggccgcac catgttcgtg ttcctggtac
     1441 tccttccttt ggtgtcttcc cagtgtgtaa atcttaccac tcggacccag cttccacccg
     1501 cctacaccaa cagtttcaca cgcggcgtct actatcctga caaggtgttt aggagttcag
     1561 tcttgcactc aactcaagac ttgttcctcc ctttctttag caatgtgacg tggtttcatg
     1621 ccatttccgg cacaaacgga acgaagcgct ttgataatcc tgtgctcccg ttcaacgatg
     1681 gagtgtactt cgcgtccaca gagaagagca atatcattcg aggttggatc ttcggaacga
     1741 cactcgactc aaagacgcag tcccttctca tcgtcaataa tgccacgaac gtggtcatca
     1801 aagtgtgcga gtttcaattc tgtaatgatc ccttcctggg cgtctatcac aagaacaaca
     1861 aatcctggat ggagtccgaa tttagagtct actccagcgc caacaactgc actttcgaat
     1921 acgtatcaca gccattcttg atggaccttg aaggaaagca gggtaatttc aagaacttga
     1981 gggagttcgt attcaagaat atcgacgggt actttaagat ttatagcaaa cacacaccca
     2041 ttaatttggt gcgggatctt cctcagggat ttagtgctct tgagcctctc gttgacctcc
     2101 ctattggcat taacatcacc cgctttcaaa ccctgttggc cctgcatcgg tcctacctga
     2161 caccgggcga ctcaagttcc ggatggaccg caggtgccgc cgcatactat gtgggctacc
     2221 ttcagccaag aacatttctg ctgaaatata atgagaacgg gaccattaca gatgcggtgg
     2281 attgtgcact cgaccctctg tctgagacga aatgcaccct taaaagcttc acggtggaga
     2341 aaggcattta tcagacttct aacttcagag ttcaacccac cgagtccatt gtgcgattcc
     2401 caaatattac gaatttgtgc ccatttggtg aggtcttcaa tgctactcga ttcgcctcag
     2461 tttatgcatg gaaccgaaag agaatttcca attgtgtggc ggactactca gtattgtata
     2521 atagtgcgag ctttagcaca ttcaaatgtt acggcgtgtc tccaacgaag ctgaacgatc
     2581 tctgtttcac aaacgtttat gcggattcct tcgtgattcg cggcgatgag gtccgacaga
     2641 ttgcgcctgg gcaaacgggt aagatcgctg attacaacta taagttgccg gacgatttca
     2701 caggatgtgt catagcttgg aatagcaata atttggacag taaggttggc ggaaactaca
     2761 attatttgta caggttgttt cgcaagtcaa atttgaaacc atttgagaga gatatatcta
     2821 cggagatata tcaagccggc tctacaccat gtaatggtgt ggagggcttt aactgctact
     2881 ttccactcca gtcatatggt ttccaaccta catatggagt agggtatcaa ccgtacagag
     2941 ttgtggtctt gagtttcgaa ttgctccacg ctccagcaac ggtatgcggt cctaagaaat
     3001 ccacaaatct tgtgaagaac aagtgcgtaa atttcaactt caatgggctg actggtaccg
     3061 gcgtgctgac tgagagtaac aagaagttct tgcctttcca acaattcggg cgggacatag
     3121 acgataccac tgacgccgtc cgcgaccctc agaccctgga gattctggac ataactcctt
     3181 gttctttcgg tggcgtcagt gttatcactc ccgggaccaa cacctccaac caagtcgcgg
     3241 tcctctatca aggcgtcaac tgtacggaag taccggtagc catccatgcg gaccaactta
     3301 caccgacttg gagggtttac tctacaggaa gcaatgtctt tcaaacacga gccgggtgtc
     3361 tgatcggagc agaacacgtt aacaacagct acgaatgtga cataccaata ggcgcaggga
     3421 tttgtgcttc atatcagaca cagaccaata gccaccggag agcgagaagc gtagcaagcc
     3481 aaagcatcat cgcgtacacg atgagcctcg gagcagagaa cagcgtcgcg tatagcaata
     3541 attcaatagc tatcccaatc aatttcacta tttcggtcac cactgagatt ctgccggtct
     3601 ccatgaccaa gacatccgtc gattgtacta tgtacatatg cggcgacagc acggagtgca
     3661 gtaacttgct ccttcagtac ggttccttct gtacgcagct taaccgggca ctgacgggta
     3721 tcgcggtaga acaggacaag aacacacagg aggtcttcgc gcaggtcaaa caaatctaca
     3781 agacaccacc cataaaggac ttcggcgggt tcaatttcag ccaaatcctg ccggaccctt
     3841 ccaaacctag taagaggtca ttcattgagg atcttctgtt taacaaagtt acgcttgcgg
     3901 acgcgggatt cattaagcag tatggtgact gccttggaga tattgccgcc agggatttga
     3961 tatgtgcaca gaaatttaac ggcctcaccg ttctgccgcc tctgctcacc gatgagatga
     4021 tagcgcagta cacgagcgca ctcctggcag gtacaattac aagcggatgg acattcggtg
     4081 caggagcagc gttgcagata ccctttgcta tgcagatggc ttatcgattt aacgggattg
     4141 gcgtcacgca gaacgtcctt tatgagaatc agaaattgat tgcaaatcag ttcaatagtg
     4201 ctatcggtaa gattcaggac agcttgagca gtaccgctag cgcactggga aagttgcagg
     4261 acgtggtgaa tcagaatgca caagcactga ataccttggt taagcaattg agtagcaatt
     4321 tcggcgccat atcaagtgta ctgaatgata tcctggctcg gttggacaag gtagaagccg
     4381 aagttcagat tgaccgcttg atcaccgggc gcctccaaag tctgcagacc tacgtcacac
     4441 aacaattgat cagagcagca gagataagag catctgctaa cctggctgcc actaagatgt
     4501 ctgaatgtgt gcttgggcag tcaaagaggg tagatttctg cggaaagggc taccacctta
     4561 tgtctttccc tcagagcgct ccgcatggtg tggtctttct ccatgtgact tatgtgcctg
     4621 ctcaagagaa gaactttacg acggcgcccg ctatatgcca tgatggtaag gcgcactttc
     4681 caagggaggg agtgttcgtg tccaacggca ctcactggtt tgtcacccaa cgaaatttct
     4741 acgagcctca aattattacc acccacaata cctttgttag cggtaactgt gacgtcgtaa
     4801 ttgggattgt taataataca gtctacgatc ctctgcagcc ggaactggac tcctttaaag
     4861 aggagctgga caaatatttc aagaaccaca catctcctga cgtagatctt ggagacataa
     4921 gcggtataaa tgcaagtgtt gttaacattc agaaagaaat agataggttg aacgaagttg
     4981 cgaagaacct taacgagtca ctgatagacc tccaagagct tgggaagtac gagcaatata
     5041 tcaagtggcc ttggtatatt tggctcgggt tcatagcagg acttatcgct atagtcatgg
     5101 tgactataat gctgtgctgc atgacaagct gctgcagctg tctcaaaggc tgttgctctt
     5161 gcggctcttg ctgcaaattc gatgaggacg attccgaacc tgtcttgaag ggtgtcaagc
     5221 tccattacac ataactcgag ggcccagatc taattcaccc caccagtgca ggctgcctat
     5281 cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta agctcgcttt
     5341 cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta ctaaactggg
     5401 ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat ttattttcat
     5461 tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg tgggaggtca
     5521 gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa tacactatat
     5581 cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg caacagcccc
     5641 tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt gatttggagg
     5701 ttaaagtttt gctatgctgt attttacatt acttattgtt ttagctgtcc tcatgaatgt
     5761 cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact cagttctctt
     5821 gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc gagatggttt
     5881 ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt ctacttgaag
     5941 aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct gcctccccca
     6001 ctcacagtga cccggaatcc ctcgacatgg cagtctagat cattcttgaa gacgaaaggg
     6061 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     6121 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6181 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6241 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6301 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6361 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6421 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6481 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6541 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6601 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6661 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6721 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6781 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6841 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6901 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6961 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7021 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7081 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7141 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7201 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7261 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7321 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7381 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7441 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7501 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7561 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7621 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7681 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7741 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7801 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7861 cctatggaaa aacgccagca acggatgcgc cgcgtgcggc tgctggagat ggcggacgcg
     7921 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg
     7981 gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg
     8041 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg
     8101 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atccccgtga
     8161 cgatcagcgg tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct
     8221 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     8281 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcggggagc
     8341 tttttgcaaa ag
//
