LOCUS       HDM_BA.2.75.2_Sp        8367 bp DNA     circular SYN 08-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported May 8, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="color: #------"
     CDS             1422..5243
                     /codon_start=1
                     /label=SARS_CoV-2_BA.2.75.2_Spike_cDNA
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNLITRTQSYTNSFTRGVYYPDKVFRSSVL
                     HSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFG
                     TTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLDVYYHENNKSRMESELRVYSSANNC
                     TFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPVNLGREPEDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSSWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFHE
                     VFNATTFASVYAWNRKRISNCVADYSVLYNFAPFFAFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGNEVSQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNKLDSKVSGNYNYLYRLFRK
                     SKLKPFERDISTEIYQAGNKPCNGVAGSNCYFPLQSYGFRPTYGVGHQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTKSHRRARSVASQSIIA
                     YTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLL
                     LQYGSFCTQLKRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKYFGGFNFSQILPDPSKP
                     SKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIA
                     QYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSA
                     IGKIQDSLSSTASALGKLQDVVNHNAQALNTLVKQLSSKFGAISSVLNDILSRLDKVEA
                     EVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYH
                     LMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR
                     NFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDL
                     GDISGINASVVNIQKEIDRLNEVAKNLNESLINLQELGKYEQYIKWPWYIWLGFIAGLI
                     AIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     polyA_signal    5347..5741
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6161..6265
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6266..7126
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6266 .. 6334 / #ccffcc / signal sequence
                        2: 6335 .. 7126 / #ccffcc
                     Cleavage site after base 6334"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7297..7885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattccagt gcggccgcac catgttcgtg ttcctggtac
     1441 tccttccttt ggtgtcttcc cagtgtgtaa atcttattac tcggacccag tcttacacca
     1501 acagtttcac acgcggcgtc tactatcctg acaaggtgtt taggagttca gtcttgcact
     1561 caactcaaga cttgttcctc cctttcttta gcaatgtgac gtggtttcat gccattcatg
     1621 tctccggcac aaacggaacg aagcgctttg ataatcctgt gctcccgttc aacgatggag
     1681 tgtacttcgc gtccacagag aagagcaata tcattcgagg ttggatcttc ggaacgacac
     1741 tcgactcaaa gacgcagtcc cttctcatcg tcaataatgc cacgaacgtg gtcatcaaag
     1801 tgtgcgagtt tcaattctgt aatgatccct tcctggatgt ctattatcac gagaacaaca
     1861 aatccagaat ggagtccgaa ctcagagtct actccagcgc caacaactgc actttcgaat
     1921 acgtatcaca gccattcttg atggaccttg aaggaaagca gggtaatttc aagaacttga
     1981 gggagttcgt attcaagaat atcgacgggt actttaagat ctatagcaaa cacacacccg
     2041 tcaatttggg ccgggaacca gaggatcttc ctcagggatt tagtgctctt gagcctctcg
     2101 ttgacctccc tattggcatt aacatcaccc gctttcaaac cctgttggcc ctgcatcggt
     2161 cctacctgac accgggcgac tcaagttcca gctggaccgc aggtgccgcc gcatactatg
     2221 tgggctacct tcagccaaga acatttctgc tgaaatataa tgagaacggg accattacag
     2281 atgcggtgga ttgtgcactc gaccctctgt ctgagacgaa atgcaccctt aaaagcttca
     2341 cggtggagaa aggcatttat cagacttcta acttcagagt tcaacccacc gagtccattg
     2401 tgcgattccc aaatattacg aatttgtgcc catttcacga ggtcttcaat gctactactt
     2461 tcgcctcagt ttacgcatgg aaccgaaaga gaatttccaa ctgtgtggcg gactactcag
     2521 tattgtataa ttttgccccg ttttttgcct tcaaatgtta cggcgtgtct ccaacgaagc
     2581 tgaacgatct ctgtttcaca aacgtttatg cggattcctt cgtgattcgc ggcaatgagg
     2641 tctctcagat tgcgcctggg caaacgggta atatcgctga ttacaactat aagttgccgg
     2701 acgatttcac aggatgtgtc atagcttgga atagcaataa gttggacagt aaggttagcg
     2761 gaaactacaa ttatttgtac aggttgtttc gcaagtcaaa gttgaaacca tttgagagag
     2821 atatatctac ggagatatat caagccggca ataaaccatg taatggtgtg gccggcagca
     2881 actgctactt tccactccag tcatatggtt tccggcctac atacggagta gggcaccaac
     2941 cgtacagagt tgtggtcttg agtttcgaat tgctccacgc tccagcaacg gtatgcggtc
     3001 ctaagaaatc cacaaatctt gtgaagaaca agtgcgtaaa tttcaacttc aatgggctga
     3061 ctggtaccgg cgtgctgact gagagtaaca agaagttctt gcctttccaa caattcgggc
     3121 gggacatagc tgataccact gacgccgtcc gcgaccctca gaccctggag attctggaca
     3181 taactccttg ttctttcggt ggcgtcagtg ttatcactcc cgggaccaac acctccaacc
     3241 aagtcgcggt cctctatcaa ggcgtcaact gtacggaagt accggtagcc atccatgcgg
     3301 accaacttac accgacttgg agggtttact ctacaggaag caatgtcttt caaacacgag
     3361 ccgggtgtct gatcggagca gaatacgtta acaacagcta cgaatgtgac ataccaatag
     3421 gcgcagggat ttgtgcttca tatcagacac agaccaaaag ccaccggaga gcgagaagcg
     3481 tagcaagcca aagcatcatc gcgtacacga tgagcctcgg agcagagaac agcgtcgcgt
     3541 atagcaataa ttcaatagct atcccaacaa atttcactat ttcggtcacc actgagattc
     3601 tgccggtctc catgaccaag acatccgtcg attgtactat gtacatatgc ggcgacagca
     3661 cggagtgcag taacttgctc cttcagtacg gttccttctg tacgcagctt aaacgggcac
     3721 tgacgggtat cgcggtagaa caggacaaga acacacagga ggtcttcgcg caggtcaaac
     3781 aaatctacaa gacaccaccc ataaagtact tcggcgggtt caatttcagc caaatcctgc
     3841 cggacccttc caaacctagt aagaggtcat tcattgagga tcttctgttt aacaaagtta
     3901 cgcttgcgga cgcgggattc attaagcagt atggtgactg ccttggagat attgccgcca
     3961 gggatttgat atgtgcacag aaatttaacg gcctcaccgt tctgccgcct ctgctcaccg
     4021 atgagatgat agcgcagtac acgagcgcac tcctggcagg tacaattaca agcggatgga
     4081 cattcggtgc aggagcagcg ttgcagatac cctttgctat gcagatggct tatcgattta
     4141 acgggattgg cgtcacgcag aacgtccttt atgagaatca gaaattgatt gcaaatcagt
     4201 tcaatagtgc tatcggtaag attcaggaca gcttgagcag taccgctagc gcactgggaa
     4261 agttgcagga cgtggtgaat cacaatgcac aagcactgaa taccttggtt aagcaattga
     4321 gtagcaaatt cggcgccata tcaagtgtac tgaatgatat cctgtcacgg ttggacaagg
     4381 tagaagccga agttcagatt gaccgcttga tcaccgggcg cctccaaagt ctgcagacct
     4441 acgtcacaca acaattgatc agagcagcag agataagagc atctgctaac ctggctgcca
     4501 ctaagatgtc tgaatgtgtg cttgggcagt caaagagggt agatttctgc ggaaagggct
     4561 accaccttat gtctttccct cagagcgctc cgcatggtgt ggtctttctc catgtgactt
     4621 atgtgcctgc tcaagagaag aactttacga cggcgcccgc tatatgccat gatggtaagg
     4681 cgcactttcc aagggaggga gtgttcgtgt ccaacggcac tcactggttt gtcacccaac
     4741 gaaatttcta cgagcctcaa attattacca ccgacaatac ctttgttagc ggtaactgtg
     4801 acgtcgtaat tgggattgtt aataatacag tctacgatcc tctgcagccg gaactggact
     4861 cctttaaaga ggagctggac aaatatttca agaaccacac atctcctgac gtagatcttg
     4921 gagacataag cggtataaat gcaagtgttg ttaacattca gaaagaaata gataggttga
     4981 acgaagttgc gaagaacctt aacgagtcac tgataaacct ccaagagctt gggaagtacg
     5041 agcaatatat caagtggcct tggtatattt ggctcgggtt catagcagga cttatcgcta
     5101 tagtcatggt gactataatg ctgtgctgca tgacaagctg ctgcagctgt ctcaaaggct
     5161 gttgctcttg cggctcttgc tgcaaattcg atgaggacga ttccgaacct gtcttgaagg
     5221 gtgtcaagct ccattacaca taacaattgc tcgagggccc agatctaatt caccccacca
     5281 gtgcaggctg cctatcagaa agtggtggct ggtgtggcta atgccctggc ccacaagtat
     5341 cactaagctc gctttcttgc tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc
     5401 aactactaaa ctgggggata ttatgaaggg ccttgagcat ctggattctg cctaataaaa
     5461 aacatttatt ttcattgcaa tgatgtattt aaattatttc tgaatatttt actaaaaagg
     5521 gaatgtggga ggtcagtgca tttaaaacat aaagaaatga agagctagtt caaaccttgg
     5581 gaaaatacac tatatcttaa actccatgaa agaaggtgag gctgcaaaca gctaatgcac
     5641 attggcaaca gcccctgatg cctatgcctt attcatccct cagaaaagga ttcaagtaga
     5701 ggcttgattt ggaggttaaa gttttgctat gctgtatttt acattactta ttgttttagc
     5761 tgtcctcatg aatgtctttt cactacccat ttgcttatcc tgcatctctc agccttgact
     5821 ccactcagtt ctcttgctta gagataccac ctttcccctg aagtgttcct tccatgtttt
     5881 acggcgagat ggtttctcct cgcctggcca ctcagcctta gttgtctctg ttgtcttata
     5941 gaggtctact tgaagaagga aaaacagggg gcatggtttg actgtcctgt gagcccttct
     6001 tccctgcctc ccccactcac agtgacccgg aatccctcga catggcagtc tagatcattc
     6061 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     6121 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6181 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6241 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6301 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6361 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6421 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6481 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6541 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6601 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6661 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6721 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6781 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6841 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6901 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6961 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7021 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7081 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7141 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7201 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgga tgcgccgcgt gcggctgctg
     7921 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     7981 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     8041 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     8101 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg
     8161 cataaatccc cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga
     8221 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     8281 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     8341 ctcgcgtcgg ggagcttttt gcaaaag
//
