LOCUS       HDM_BA.2.75_Spik        8362 bp DNA     circular SYN 08-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported May 8, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="color: #------"
     CDS             1423..5244
                     /codon_start=1
                     /label=SARS_CoV-2_BA.2.75_Spike
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNLITRTQSYTNSFTRGVYYPDKVFRSSVL
                     HSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFG
                     TTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLDVYYHENNKSRMESELRVYSSANNC
                     TFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPVNLGREPEDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSSWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFHE
                     VFNATRFASVYAWNRKRISNCVADYSVLYNFAPFFAFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGNEVSQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNKLDSKVSGNYNYLYRLFRK
                     SKLKPFERDISTEIYQAGNKPCNGVAGFNCYFPLQSYGFRPTYGVGHQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTKSHRRARSVASQSIIA
                     YTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLL
                     LQYGSFCTQLKRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKYFGGFNFSQILPDPSKP
                     SKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIA
                     QYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSA
                     IGKIQDSLSSTASALGKLQDVVNHNAQALNTLVKQLSSKFGAISSVLNDILSRLDKVEA
                     EVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYH
                     LMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR
                     NFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDL
                     GDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLI
                     AIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     polyA_signal    5342..5736
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6156..6260
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6261..7121
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6261 .. 6329 / #ccffcc / signal sequence
                        2: 6330 .. 7121 / #ccffcc
                     Cleavage site after base 6329"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7292..7880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattccgcg ggcggccgca ccatgttcgt gttcctggta
     1441 ctccttcctt tggtgtcttc ccagtgtgta aatcttatta ctcggaccca gtcttacacc
     1501 aacagtttca cacgcggcgt ctactatcct gacaaggtgt ttaggagttc agtcttgcac
     1561 tcaactcaag acttgttcct ccctttcttt agcaatgtga cgtggtttca tgccattcat
     1621 gtctccggca caaacggaac gaagcgcttt gataatcctg tgctcccgtt caacgatgga
     1681 gtgtacttcg cgtccacaga gaagagcaat atcattcgag gttggatctt cggaacgaca
     1741 ctcgactcaa agacgcagtc ccttctcatc gtcaataatg ccacgaacgt ggtcatcaaa
     1801 gtgtgcgagt ttcaattctg taatgatccc ttcctggatg tctattatca cgagaacaac
     1861 aaatccagaa tggagtccga actcagagtc tactccagcg ccaacaactg cactttcgaa
     1921 tacgtatcac agccattctt gatggacctt gaaggaaagc agggtaattt caagaacttg
     1981 agggagttcg tattcaagaa tatcgacggg tactttaaga tctatagcaa acacacaccc
     2041 gtcaatttgg gccgggaacc agaggatctt cctcagggat ttagtgctct tgagcctctc
     2101 gttgacctcc ctattggcat taacatcacc cgctttcaaa ccctgttggc cctgcatcgg
     2161 tcctacctga caccgggcga ctcaagttcc agctggaccg caggtgccgc cgcatactat
     2221 gtgggctacc ttcagccaag aacatttctg ctgaaatata atgagaacgg gaccattaca
     2281 gatgcggtgg attgtgcact cgaccctctg tctgagacga aatgcaccct taaaagcttc
     2341 acggtggaga aaggcattta tcagacttct aacttcagag ttcaacccac cgagtccatt
     2401 gtgcgattcc caaatattac gaatttgtgc ccatttcacg aggtcttcaa tgctactcga
     2461 ttcgcctcag tttacgcatg gaaccgaaag agaatttcca actgtgtggc ggactactca
     2521 gtattgtata attttgcccc gttttttgcc ttcaaatgtt acggcgtgtc tccaacgaag
     2581 ctgaacgatc tctgtttcac aaacgtttat gcggattcct tcgtgattcg cggcaatgag
     2641 gtctctcaga ttgcgcctgg gcaaacgggt aatatcgctg attacaacta taagttgccg
     2701 gacgatttca caggatgtgt catagcttgg aatagcaata agttggacag taaggttagc
     2761 ggaaactaca attatttgta caggttgttt cgcaagtcaa agttgaaacc atttgagaga
     2821 gatatatcta cggagatata tcaagccggc aataaaccat gtaatggtgt ggccggcttt
     2881 aactgctact ttccactcca gtcatatggt ttccggccta catacggagt agggcaccaa
     2941 ccgtacagag ttgtggtctt gagtttcgaa ttgctccacg ctccagcaac ggtatgcggt
     3001 cctaagaaat ccacaaatct tgtgaagaac aagtgcgtaa atttcaactt caatgggctg
     3061 actggtaccg gcgtgctgac tgagagtaac aagaagttct tgcctttcca acaattcggg
     3121 cgggacatag ctgataccac tgacgccgtc cgcgaccctc agaccctgga gattctggac
     3181 ataactcctt gttctttcgg tggcgtcagt gttatcactc ccgggaccaa cacctccaac
     3241 caagtcgcgg tcctctatca aggcgtcaac tgtacggaag taccggtagc catccatgcg
     3301 gaccaactta caccgacttg gagggtttac tctacaggaa gcaatgtctt tcaaacacga
     3361 gccgggtgtc tgatcggagc agaatacgtt aacaacagct acgaatgtga cataccaata
     3421 ggcgcaggga tttgtgcttc atatcagaca cagaccaaaa gccaccggag agcgagaagc
     3481 gtagcaagcc aaagcatcat cgcgtacacg atgagcctcg gagcagagaa cagcgtcgcg
     3541 tatagcaata attcaatagc tatcccaaca aatttcacta tttcggtcac cactgagatt
     3601 ctgccggtct ccatgaccaa gacatccgtc gattgtacta tgtacatatg cggcgacagc
     3661 acggagtgca gtaacttgct ccttcagtac ggttccttct gtacgcagct taaacgggca
     3721 ctgacgggta tcgcggtaga acaggacaag aacacacagg aggtcttcgc gcaggtcaaa
     3781 caaatctaca agacaccacc cataaagtac ttcggcgggt tcaatttcag ccaaatcctg
     3841 ccggaccctt ccaaacctag taagaggtca ttcattgagg atcttctgtt taacaaagtt
     3901 acgcttgcgg acgcgggatt cattaagcag tatggtgact gccttggaga tattgccgcc
     3961 agggatttga tatgtgcaca gaaatttaac ggcctcaccg ttctgccgcc tctgctcacc
     4021 gatgagatga tagcgcagta cacgagcgca ctcctggcag gtacaattac aagcggatgg
     4081 acattcggtg caggagcagc gttgcagata ccctttgcta tgcagatggc ttatcgattt
     4141 aacgggattg gcgtcacgca gaacgtcctt tatgagaatc agaaattgat tgcaaatcag
     4201 ttcaatagtg ctatcggtaa gattcaggac agcttgagca gtaccgctag cgcactggga
     4261 aagttgcagg acgtggtgaa tcacaatgca caagcactga ataccttggt taagcaattg
     4321 agtagcaaat tcggcgccat atcaagtgta ctgaatgata tcctgtcacg gttggacaag
     4381 gtagaagccg aagttcagat tgaccgcttg atcaccgggc gcctccaaag tctgcagacc
     4441 tacgtcacac aacaattgat cagagcagca gagataagag catctgctaa cctggctgcc
     4501 actaagatgt ctgaatgtgt gcttgggcag tcaaagaggg tagatttctg cggaaagggc
     4561 taccacctta tgtctttccc tcagagcgct ccgcatggtg tggtctttct ccatgtgact
     4621 tatgtgcctg ctcaagagaa gaactttacg acggcgcccg ctatatgcca tgatggtaag
     4681 gcgcactttc caagggaggg agtgttcgtg tccaacggca ctcactggtt tgtcacccaa
     4741 cgaaatttct acgagcctca aattattacc accgacaata cctttgttag cggtaactgt
     4801 gacgtcgtaa ttgggattgt taataataca gtctacgatc ctctgcagcc ggaactggac
     4861 tcctttaaag aggagctgga caaatatttc aagaaccaca catctcctga cgtagatctt
     4921 ggagacataa gcggtataaa tgcaagtgtt gttaacattc agaaagaaat agataggttg
     4981 aacgaagttg cgaagaacct taacgagtca ctgatagacc tccaagagct tgggaagtac
     5041 gagcaatata tcaagtggcc ttggtatatt tggctcgggt tcatagcagg acttatcgct
     5101 atagtcatgg tgactataat gctgtgctgc atgacaagct gctgcagctg tctcaaaggc
     5161 tgttgctctt gcggctcttg ctgcaaattc gatgaggacg attccgaacc tgtcttgaag
     5221 ggtgtcaagc tccattacac ataactcgag ggcccagatc taattcaccc caccagtgca
     5281 ggctgcctat cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta
     5341 agctcgcttt cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta
     5401 ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat
     5461 ttattttcat tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg
     5521 tgggaggtca gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa
     5581 tacactatat cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg
     5641 caacagcccc tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt
     5701 gatttggagg ttaaagtttt gctatgctgt attttacatt acttattgtt ttagctgtcc
     5761 tcatgaatgt cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact
     5821 cagttctctt gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc
     5881 gagatggttt ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt
     5941 ctacttgaag aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct
     6001 gcctccccca ctcacagtga cccggaatcc ctcgacatgg cagtctagat cattcttgaa
     6061 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     6121 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6181 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6241 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6301 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6361 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6421 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6481 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6541 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6601 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6661 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6721 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6781 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6841 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6901 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6961 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7021 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7081 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7141 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7201 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7861 gggggcggag cctatggaaa aacgccagca acggatgcgc cgcgtgcggc tgctggagat
     7921 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     7981 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc
     8041 attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg
     8101 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa
     8161 atccccgtga cgatcagcgg tccaatgatc gaagttaggc tggtaagagc cgcgagcgat
     8221 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
     8281 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
     8341 gtcggggagc tttttgcaaa ag
//
