LOCUS       Exported                8356 bp DNA     circular SYN 12-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Nov 12, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="color: #------"
     CDS             1423..5238
                     /codon_start=1
                     /label=SARS-CoV-2_Delta_Y145H_Spike
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNLRTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYHHKNNKSWMESGVYSSANN
                     CTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALE
                     PLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENG
                     TITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVF
                     NATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFV
                     IRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYRYRLFRKSN
                     LKPFERDISTEIYQAGSKPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLH
                     APATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRD
                     PQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYS
                     TGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSRRRARSVASQSIIAYT
                     MSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQ
                     YGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSK
                     RSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQY
                     TSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIG
                     KIQDSLSSTASALGKLQNVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEV
                     QIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLM
                     SFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNF
                     YEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGD
                     ISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAI
                     VMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     polyA_signal    5336..5730
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6150..6254
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6255..7115
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6255 .. 6323 / #ccffcc / signal sequence
                        2: 6324 .. 7115 / #ccffcc
                     Cleavage site after base 6323"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7286..7874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattccgcg ggcggccgca ccatgttcgt gttcctggta
     1441 ctccttcctt tggtgtcttc ccagtgtgta aatcttcgca ctcggaccca gcttccaccc
     1501 gcctacacca acagtttcac acgcggcgtc tactatcctg acaaggtgtt taggagttca
     1561 gtcttgcact caactcaaga cttgttcctc cctttcttta gcaatgtgac gtggtttcat
     1621 gccattcatg tctccggcac aaacggaacg aagcgctttg ataatcctgt gctcccgttc
     1681 aacgatggag tgtacttcgc gtccacagag aagagcaata tcattcgagg ttggatcttc
     1741 ggaacgacac tcgactcaaa gacgcagtcc cttctcatcg tcaataatgc cacgaacgtg
     1801 gtcatcaaag tgtgcgagtt tcaattctgt aatgatccct tcctgggcgt ctatcaccac
     1861 aagaacaaca aatcctggat ggagtccgga gtctactcca gcgccaacaa ctgcactttc
     1921 gaatacgtat cacagccatt cttgatggac cttgaaggaa agcagggtaa tttcaagaac
     1981 ttgagggagt tcgtattcaa gaatatcgac gggtacttta agatttatag caaacacaca
     2041 cccattaatt tggtgcggga tcttcctcag ggatttagtg ctcttgagcc tctcgttgac
     2101 ctccctattg gcattaacat cacccgcttt caaaccctgt tggccctgca tcggtcctac
     2161 ctgacaccgg gcgactcaag ttccggatgg accgcaggtg ccgccgcata ctatgtgggc
     2221 taccttcagc caagaacatt tctgctgaaa tataatgaga acgggaccat tacagatgcg
     2281 gtggattgtg cactcgaccc tctgtctgag acgaaatgca cccttaaaag cttcacggtg
     2341 gagaaaggca tttatcagac ttctaacttc agagttcaac ccaccgagtc cattgtgcga
     2401 ttcccaaata ttacgaattt gtgcccattt ggtgaggtct tcaatgctac tcgattcgcc
     2461 tcagtttatg catggaaccg aaagagaatt tccaattgtg tggcggacta ctcagtattg
     2521 tataatagtg cgagctttag cacattcaaa tgttacggcg tgtctccaac gaagctgaac
     2581 gatctctgtt tcacaaacgt ttatgcggat tccttcgtga ttcgcggcga tgaggtccga
     2641 cagattgcgc ctgggcaaac gggtaagatc gctgattaca actataagtt gccggacgat
     2701 ttcacaggat gtgtcatagc ttggaatagc aataatttgg acagtaaggt tggcggaaac
     2761 tacaattatc gatacaggtt gtttcgcaag tcaaatttga aaccatttga gagagatata
     2821 tctacggaga tatatcaagc cggctctaag ccatgtaatg gtgtggaggg ctttaactgc
     2881 tactttccac tccagtcata tggtttccaa cctacaaatg gagtagggta tcaaccgtac
     2941 agagttgtgg tcttgagttt cgaattgctc cacgctccag caacggtatg cggtcctaag
     3001 aaatccacaa atcttgtgaa gaacaagtgc gtaaatttca acttcaatgg gctgactggt
     3061 accggcgtgc tgactgagag taacaagaag ttcttgcctt tccaacaatt cgggcgggac
     3121 atagctgata ccactgacgc cgtccgcgac cctcagaccc tggagattct ggacataact
     3181 ccttgttctt tcggtggcgt cagtgttatc actcccggga ccaacacctc caaccaagtc
     3241 gcggtcctct atcaaggcgt caactgtacg gaagtaccgg tagccatcca tgcggaccaa
     3301 cttacaccga cttggagggt ttactctaca ggaagcaatg tctttcaaac acgagccggg
     3361 tgtctgatcg gagcagaaca cgttaacaac agctacgaat gtgacatacc aataggcgca
     3421 gggatttgtg cttcatatca gacacagacc aatagcagac ggagagcgag aagcgtagca
     3481 agccaaagca tcatcgcgta cacgatgagc ctcggagcag agaacagcgt cgcgtatagc
     3541 aataattcaa tagctatccc aacaaatttc actatttcgg tcaccactga gattctgccg
     3601 gtctccatga ccaagacatc cgtcgattgt actatgtaca tatgcggcga cagcacggag
     3661 tgcagtaact tgctccttca gtacggttcc ttctgtacgc agcttaaccg ggcactgacg
     3721 ggtatcgcgg tagaacagga caagaacaca caggaggtct tcgcgcaggt caaacaaatc
     3781 tacaagacac cacccataaa ggacttcggc gggttcaatt tcagccaaat cctgccggac
     3841 ccttccaaac ctagtaagag gtcattcatt gaggatcttc tgtttaacaa agttacgctt
     3901 gcggacgcgg gattcattaa gcagtatggt gactgccttg gagatattgc cgccagggat
     3961 ttgatatgtg cacagaaatt taacggcctc accgttctgc cgcctctgct caccgatgag
     4021 atgatagcgc agtacacgag cgcactcctg gcaggtacaa ttacaagcgg atggacattc
     4081 ggtgcaggag cagcgttgca gatacccttt gctatgcaga tggcttatcg atttaacggg
     4141 attggcgtca cgcagaacgt cctttatgag aatcagaaat tgattgcaaa tcagttcaat
     4201 agtgctatcg gtaagattca ggacagcttg agcagtaccg ctagcgcact gggaaagttg
     4261 cagaacgtgg tgaatcagaa tgcacaagca ctgaatacct tggttaagca attgagtagc
     4321 aatttcggcg ccatatcaag tgtactgaat gatatcctgt cacggttgga caaggtagaa
     4381 gccgaagttc agattgaccg cttgatcacc gggcgcctcc aaagtctgca gacctacgtc
     4441 acacaacaat tgatcagagc agcagagata agagcatctg ctaacctggc tgccactaag
     4501 atgtctgaat gtgtgcttgg gcagtcaaag agggtagatt tctgcggaaa gggctaccac
     4561 cttatgtctt tccctcagag cgctccgcat ggtgtggtct ttctccatgt gacttatgtg
     4621 cctgctcaag agaagaactt tacgacggcg cccgctatat gccatgatgg taaggcgcac
     4681 tttccaaggg agggagtgtt cgtgtccaac ggcactcact ggtttgtcac ccaacgaaat
     4741 ttctacgagc ctcaaattat taccaccgac aatacctttg ttagcggtaa ctgtgacgtc
     4801 gtaattggga ttgttaataa tacagtctac gatcctctgc agccggaact ggactccttt
     4861 aaagaggagc tggacaaata tttcaagaac cacacatctc ctgacgtaga tcttggagac
     4921 ataagcggta taaatgcaag tgttgttaac attcagaaag aaatagatag gttgaacgaa
     4981 gttgcgaaga accttaacga gtcactgata gacctccaag agcttgggaa gtacgagcaa
     5041 tatatcaagt ggccttggta tatttggctc gggttcatag caggacttat cgctatagtc
     5101 atggtgacta taatgctgtg ctgcatgaca agctgctgca gctgtctcaa aggctgttgc
     5161 tcttgcggct cttgctgcaa attcgatgag gacgattccg aacctgtctt gaagggtgtc
     5221 aagctccatt acacataact cgagggccca gatctaattc accccaccag tgcaggctgc
     5281 ctatcagaaa gtggtggctg gtgtggctaa tgccctggcc cacaagtatc actaagctcg
     5341 ctttcttgct gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac
     5401 tgggggatat tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt
     5461 tcattgcaat gatgtattta aattatttct gaatatttta ctaaaaaggg aatgtgggag
     5521 gtcagtgcat ttaaaacata aagaaatgaa gagctagttc aaaccttggg aaaatacact
     5581 atatcttaaa ctccatgaaa gaaggtgagg ctgcaaacag ctaatgcaca ttggcaacag
     5641 cccctgatgc ctatgcctta ttcatccctc agaaaaggat tcaagtagag gcttgatttg
     5701 gaggttaaag ttttgctatg ctgtatttta cattacttat tgttttagct gtcctcatga
     5761 atgtcttttc actacccatt tgcttatcct gcatctctca gccttgactc cactcagttc
     5821 tcttgcttag agataccacc tttcccctga agtgttcctt ccatgtttta cggcgagatg
     5881 gtttctcctc gcctggccac tcagccttag ttgtctctgt tgtcttatag aggtctactt
     5941 gaagaaggaa aaacaggggg catggtttga ctgtcctgtg agcccttctt ccctgcctcc
     6001 cccactcaca gtgacccgga atccctcgac atggcagtct agatcattct tgaagacgaa
     6061 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6121 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6181 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6241 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6301 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6361 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6421 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6481 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6541 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6601 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6661 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6721 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6781 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6841 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6901 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6961 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7021 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7081 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7141 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7201 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7261 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7321 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7381 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7441 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7501 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7561 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7621 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7681 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7741 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7801 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7861 ggagcctatg gaaaaacgcc agcaacggat gcgccgcgtg cggctgctgg agatggcgga
     7921 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg
     7981 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag
     8041 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg
     8101 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcccc
     8161 gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga
     8221 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
     8281 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcggg
     8341 gagctttttg caaaag
//
