LOCUS       HDM_EG.5.1_Spike        8360 bp DNA     circular SYN 19-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported May 8, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2048..2056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1418..5236
                     /codon_start=1
                     /label=SARS_CoV-2_EG.5.1_Spike_cDNA
                     /translation="MFVFLVLLPLVSSQCVNLITRTQSYTNSFTRGVYYPDKVFRSSVL
                     HSTHDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPALPFNDGVYFASTEKSNIIRGWIFG
                     TTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLDVYQKNNKSWMESEFRVYSSANNCT
                     FEYVSQPFLMDLEGKEGNFKNLREFVFKNIDGYFKIYSKHTPINLEREPEDLPQGFSAL
                     EPLVDLPIGINITRFQTLLALHRSYLTPVDSSSGWTAGAAAYYVGYLQPRTFLLKYNEN
                     GTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFHEV
                     FNATTFASVYAWNRKRISNCVADYSVIYNFAPFFAFKCYGVSPTKLNDLCFTNVYADSF
                     VIRGNEVSQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNKLDSKPSGNYNYLYRLLRKS
                     KLKPFERDISTEIYQAGNKPCNGVAGPNCYSPLQSYGFRPTYGVGHQPYRVVVLSFELL
                     HAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVR
                     DPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVY
                     STGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTKSHRRARSVASQSIIAY
                     TMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLL
                     QYGSFCTQLKRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKYFGGFNFSQILPDPSKPS
                     KRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQ
                     YTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAI
                     GKIQDSLSSTASALGKLQDVVNHNAQALNTLVKQLSSKFGAISSVLNDILSRLDKVEAE
                     VQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHL
                     MSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRN
                     FYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLG
                     DISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIA
                     IVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     polyA_signal    5340..5734
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     promoter        6154..6258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6259..6327,6328..7119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7290..7878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattcgcgg ccgcaccatg ttcgtgttcc tggtactcct
     1441 tcctttggtg tcttcccagt gtgtaaatct tatcactcgg acccagtctt acaccaacag
     1501 tttcacacgc ggcgtctact atcctgacaa ggtgtttagg agttcagtct tgcactcaac
     1561 tcacgacttg ttcctccctt tctttagcaa tgtgacgtgg tttcatgcca ttcatgtctc
     1621 cggcacaaac ggaacgaagc gctttgataa tcctgccctc ccgttcaacg atggagtgta
     1681 cttcgcgtcc acagagaaga gcaatatcat tcgaggttgg atcttcggaa cgacactcga
     1741 ctcaaagacg cagtcccttc tcatcgtcaa taatgccacg aacgtggtca tcaaagtgtg
     1801 cgagtttcaa ttctgtaatg atcccttcct ggacgtctat cagaagaaca acaaatcctg
     1861 gatggagtcc gaatttagag tctactccag cgccaacaac tgcactttcg aatacgtatc
     1921 acagccattc ttgatggacc ttgaaggaaa ggagggtaat ttcaagaact tgagggagtt
     1981 cgtattcaag aatatcgacg ggtactttaa gatttatagc aaacacacac ccattaattt
     2041 ggagcgggaa ccagaggatc ttcctcaggg atttagtgct cttgagcctc tcgttgacct
     2101 ccctattggc attaacatca cccgctttca aaccctgttg gccctgcatc ggtcctacct
     2161 gacacccgtg gactcaagtt ccggatggac cgcaggtgcc gccgcatact atgtgggcta
     2221 ccttcagcca agaacatttc tgctgaaata taatgagaac gggaccatta cagatgcggt
     2281 ggattgtgca ctcgaccctc tgtctgagac gaaatgcacc cttaaaagct tcacggtgga
     2341 gaaaggcatt tatcagactt ctaacttcag agttcaaccc accgagtcca ttgtgcgatt
     2401 cccaaatatt acgaatttgt gcccatttca cgaggtcttc aatgctacta cgttcgcctc
     2461 agtttatgca tggaaccgaa agagaatttc caattgtgtg gcggactact cagtaattta
     2521 taattttgcc cccttttttg ccttcaaatg ttacggcgtg tctccaacga agctgaacga
     2581 tctctgtttc acaaacgttt atgcggattc cttcgtgatt cgcggcaacg aggtcagcca
     2641 gattgcgcct gggcaaacgg gtaatatcgc tgattacaac tataagttgc cggacgattt
     2701 cacaggatgt gtcatagctt ggaatagcaa taaattggac agtaagccct ctggaaacta
     2761 caattatttg tacaggttgt tgcgcaagtc aaaattgaaa ccatttgaga gagatatatc
     2821 tacggagata tatcaagccg gcaacaaacc atgtaatggt gtggccggcc ccaattgcta
     2881 ctctccactc cagtcatatg gtttcaggcc tacatatgga gtagggcacc aaccgtacag
     2941 agttgtggtc ttgagtttcg aattgctcca cgctccagca acggtatgcg gtcctaagaa
     3001 atccacaaat cttgtgaaga acaagtgcgt aaatttcaac ttcaatgggc tgactggtac
     3061 cggcgtgctg actgagagta acaagaagtt cttgcctttc caacaattcg ggcgggacat
     3121 agctgatacc actgacgccg tccgcgaccc tcagaccctg gagattctgg acataactcc
     3181 ttgttctttc ggtggcgtca gtgttatcac tcccgggacc aacacctcca accaagtcgc
     3241 ggtcctctat caaggcgtca actgtacgga agtaccggta gccatccatg cggaccaact
     3301 tacaccgact tggagggttt actctacagg aagcaatgtc tttcaaacac gagccgggtg
     3361 tctgatcgga gcagaatatg ttaacaacag ctacgaatgt gacataccaa taggcgcagg
     3421 gatttgtgct tcatatcaga cacagaccaa aagccaccgg agagcgagaa gcgtagcaag
     3481 ccaaagcatc atcgcgtaca cgatgagcct cggagcagag aacagcgtcg cgtatagcaa
     3541 taattcaata gctatcccaa caaatttcac tatttcggtc accactgaga ttctgccggt
     3601 ctccatgacc aagacatccg tcgattgtac tatgtacata tgcggcgaca gcacggagtg
     3661 cagtaacttg ctccttcagt acggttcctt ctgtacgcag cttaaacggg cactgacggg
     3721 tatcgcggta gaacaggaca agaacacaca ggaggtcttc gcgcaggtca aacaaatcta
     3781 caagacacca cccataaagt atttcggcgg gttcaatttc agccaaatcc tgccggaccc
     3841 ttccaaacct agtaagaggt cattcattga ggatcttctg tttaacaaag ttacgcttgc
     3901 ggacgcggga ttcattaagc agtatggtga ctgccttgga gatattgccg ccagggattt
     3961 gatatgtgca cagaaattta acggcctcac cgttctgccg cctctgctca ccgatgagat
     4021 gatagcgcag tacacgagcg cactcctggc aggtacaatt acaagcggat ggacattcgg
     4081 tgcaggagca gcgttgcaga taccctttgc tatgcagatg gcttatcgat ttaacgggat
     4141 tggcgtcacg cagaacgtcc tttatgagaa tcagaaattg attgcaaatc agttcaatag
     4201 tgctatcggt aagattcagg acagcttgag cagtaccgct agcgcactgg gaaagttgca
     4261 ggacgtggtg aatcacaatg cacaagcact gaataccttg gttaagcaat tgagtagcaa
     4321 gttcggcgcc atatcaagtg tactgaatga tatcctgtca cggttggaca aggtagaagc
     4381 cgaagttcag attgaccgct tgatcaccgg gcgcctccaa agtctgcaga cctacgtcac
     4441 acaacaattg atcagagcag cagagataag agcatctgct aacctggctg ccactaagat
     4501 gtctgaatgt gtgcttgggc agtcaaagag ggtagatttc tgcggaaagg gctaccacct
     4561 tatgtctttc cctcagagcg ctccgcatgg tgtggtcttt ctccatgtga cttatgtgcc
     4621 tgctcaagag aagaacttta cgacggcgcc cgctatatgc catgatggta aggcgcactt
     4681 tccaagggag ggagtgttcg tgtccaacgg cactcactgg tttgtcaccc aacgaaattt
     4741 ctacgagcct caaattatta ccaccgacaa tacctttgtt agcggtaact gtgacgtcgt
     4801 aattgggatt gttaataata cagtctacga tcctctgcag ccggaactgg actcctttaa
     4861 agaggagctg gacaaatatt tcaagaacca cacatctcct gacgtagatc ttggagacat
     4921 aagcggtata aatgcaagtg ttgttaacat tcagaaagaa atagataggt tgaacgaagt
     4981 tgcgaagaac cttaacgagt cactgataga cctccaagag cttgggaagt acgagcaata
     5041 tatcaagtgg ccttggtata tttggctcgg gttcatagca ggacttatcg ctatagtcat
     5101 ggtgactata atgctgtgct gcatgacaag ctgctgcagc tgtctcaaag gctgttgctc
     5161 ttgcggctct tgctgcaaat tcgatgagga cgattccgaa cctgtcttga agggtgtcaa
     5221 gctccattac acataacaat tgctcgaggg cccagatcta attcacccca ccagtgcagg
     5281 ctgcctatca gaaagtggtg gctggtgtgg ctaatgccct ggcccacaag tatcactaag
     5341 ctcgctttct tgctgtccaa tttctattaa aggttccttt gttccctaag tccaactact
     5401 aaactggggg atattatgaa gggccttgag catctggatt ctgcctaata aaaaacattt
     5461 attttcattg caatgatgta tttaaattat ttctgaatat tttactaaaa agggaatgtg
     5521 ggaggtcagt gcatttaaaa cataaagaaa tgaagagcta gttcaaacct tgggaaaata
     5581 cactatatct taaactccat gaaagaaggt gaggctgcaa acagctaatg cacattggca
     5641 acagcccctg atgcctatgc cttattcatc cctcagaaaa ggattcaagt agaggcttga
     5701 tttggaggtt aaagttttgc tatgctgtat tttacattac ttattgtttt agctgtcctc
     5761 atgaatgtct tttcactacc catttgctta tcctgcatct ctcagccttg actccactca
     5821 gttctcttgc ttagagatac cacctttccc ctgaagtgtt ccttccatgt tttacggcga
     5881 gatggtttct cctcgcctgg ccactcagcc ttagttgtct ctgttgtctt atagaggtct
     5941 acttgaagaa ggaaaaacag ggggcatggt ttgactgtcc tgtgagccct tcttccctgc
     6001 ctcccccact cacagtgacc cggaatccct cgacatggca gtctagatca ttcttgaaga
     6061 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6121 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6181 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6241 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6301 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6361 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6421 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6481 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6541 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6601 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6661 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6721 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6781 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6841 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6901 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6961 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7021 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7081 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7141 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7201 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7261 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7321 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7381 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7441 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7501 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7561 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7621 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7681 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7741 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7801 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7861 gggcggagcc tatggaaaaa cgccagcaac ggatgcgccg cgtgcggctg ctggagatgg
     7921 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
     7981 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat
     8041 tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
     8101 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
     8161 ccccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
     8221 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
     8281 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
     8341 cggggagctt tttgcaaaag
//
