LOCUS       HDM_KP.2_Spike        8349 bp DNA     circular SYN 10-MAY-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Sep 23, 2024 from SnapGene 7.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8349
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="color: #------"
     CDS             1422..5231
                     /codon_start=1
                     /label=SARS_CoV-2_KP.2_Spike_cDNA
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVMPLFNLITTTQSYTNSFTRGVYYPDKVFR
                     SSVLHLTQDLFLPFFSNVTWFHAISGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWI
                     FGTTLDSKTQSLLIVNNATNVFIKVCEFQFCNDPFLDVYHKNNKSWMESESGVYSSANN
                     CTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPIIGRDFPQGFSALEP
                     LVDLPIGINITRFQTLLALNRSYLTPGDSSSGWTAGAADYYVGYLQPRTFLLKYNENGT
                     ITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNVTNLCPFHEVFN
                     ATTFASVYAWNRTRISNCVADYSVLYNFAPFFAFKCYGVSPTKLNDLCFTNVYADSFVI
                     KGNEVSQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNKLDSKHSGNYDYWYRSLRKSKL
                     KPFERDISTEIYQAGNKPCKGKGPNCYFPLQSYGFRPTYGVGHQPYRVVVLSFELLHAP
                     ATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTKSNKKFLPFQQFGRDIVDTTDAVRDPQ
                     TLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVSVAIHADQLTPTWRVYSTG
                     SNVFQTRAGCLIGAEYVNNSYECDIPIGAGVCASYQTQTKSRRRARSVASQSIIAYTMS
                     LGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYG
                     SFCTQLKRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKYFGGFNFSQILPDPSKPSKRS
                     FIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTS
                     ALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKI
                     QDSLFSTASALGKLQDVVNHNAQALNTLVKQLSSKFGAISSVLNDILSRLDKVEAEVQI
                     DRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSF
                     PQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYE
                     PQIITTDNTFVSGNCDVVIGIVNNTVYDPLQLELDSFKEELDKYFKNHTSPDVDLGDIS
                     GINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVM
                     VTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     CDS             2330..3050
                     /label=KP.2_RBD
                     /note="color: #339966; direction: RIGHT"
     polyA_signal    5329..5723
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6143..6247
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6248..7108
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6248 .. 6316 / #ccffcc / signal sequence
                        2: 6317 .. 7108 / #ccffcc
                     Cleavage site after base 6316"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7279..7867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattccagt gcggccgcac catgttcgtg ttcctggtac
     1441 tccttccttt ggtgtcttcc cagtgtgtaa tgcccctctt taatcttatt actactaccc
     1501 agtcctacac caacagtttc acacgcggcg tctactatcc tgacaaggtg tttaggagtt
     1561 cagtcttgca cctcactcaa gacttgttcc tccctttctt tagcaatgtg acgtggtttc
     1621 atgccatttc cggcacaaac ggaacgaagc gctttgataa tcctgtgctc ccgttcaacg
     1681 atggagtgta cttcgcgtcc acagagaaga gcaatatcat tcgaggttgg atcttcggaa
     1741 cgacactcga ctcaaagacg cagtcccttc tcatcgtcaa taatgccacg aacgtgttta
     1801 tcaaagtgtg cgagtttcaa ttctgtaatg atcccttcct ggacgtctat cacaagaaca
     1861 acaaatcctg gatggagtcc gaatcagggg tctactccag cgccaacaac tgcactttcg
     1921 aatacgtatc acagccattc ttgatggacc ttgaaggaaa gcagggtaat ttcaagaact
     1981 tgagggagtt cgtattcaag aatatcgacg ggtactttaa gatttatagc aaacacacac
     2041 ccattatcgg gcgggatttt cctcagggat ttagtgctct tgagcctctc gttgacctcc
     2101 ctattggcat taacatcacc cgctttcaaa ccctgttggc cctgaatcgg tcctacctga
     2161 caccgggcga ctcaagttcc ggatggaccg caggtgccgc cgactactat gtgggctacc
     2221 ttcagccaag aacatttctg ctgaaatata atgagaacgg gaccattaca gatgcggtgg
     2281 attgtgcact cgaccctctg tctgagacga aatgcaccct taaaagcttc acggtggaga
     2341 aaggcattta tcagacttct aacttcagag ttcaacccac cgaatcgatt gtgcgattcc
     2401 caaatgtgac gaatttgtgc ccatttcacg aggtcttcaa tgctacgact ttcgcctcag
     2461 tttatgcatg gaaccgaacg agaatttcca attgtgtggc ggactactca gtattgtata
     2521 attttgcccc cttttttgca ttcaaatgtt acggcgtgtc tccaacgaag ctgaacgatc
     2581 tctgtttcac aaacgtttat gcggattcct tcgtgattaa aggcaacgag gtctcccaga
     2641 ttgcgcctgg gcaaacgggt aacatcgctg attacaacta taagttgccg gacgatttca
     2701 caggatgtgt catagcttgg aatagcaata aattggacag taagcactca ggaaactacg
     2761 attattggta caggagtctc cgcaagtcaa aattgaaacc atttgagaga gatatatcta
     2821 cggagatata tcaagccggc aataaaccat gtaaaggtaa aggccctaac tgctactttc
     2881 cactccagtc atatggtttc aggcctacat atggagtagg gcaccaaccg tacagagttg
     2941 tggtcttgag tttcgaattg ctccacgctc cagcaacggt atgcggtcct aagaagtcga
     3001 caaatcttgt gaagaacaag tgcgtaaatt tcaacttcaa tgggctgact ggtaccggcg
     3061 tgctgactaa aagtaacaag aagttcttgc ctttccaaca attcgggcgg gacatagtgg
     3121 ataccactga cgccgtccgc gaccctcaga ccctggagat tctggacata actccttgtt
     3181 ctttcggtgg cgtcagtgtt atcactcccg ggaccaacac ctccaaccaa gtcgcggtcc
     3241 tctatcaagg ggtcaactgt accgaagtat ctgtagccat ccatgcggac caacttacac
     3301 cgacttggag ggtttactct acaggaagca atgtctttca aacacgagcc gggtgtctga
     3361 tcggagcaga atacgttaac aacagctacg aatgtgacat accaataggc gcaggggttt
     3421 gtgcttcata tcagacacag accaaaagca ggcggagagc gagaagcgta gcaagccaaa
     3481 gcatcatcgc gtacacgatg agcctcggag cagagaacag cgtcgcgtat agcaataatt
     3541 caatagctat cccaacaaat ttcactattt cggtcaccac tgagattctg ccggtctcca
     3601 tgaccaagac atccgtcgat tgtactatgt acatatgcgg cgacagcacg gagtgcagta
     3661 acttgctcct tcagtacggt tccttctgta cgcagcttaa acgggcactg acgggtatcg
     3721 cggtagaaca ggacaagaac acacaggagg tcttcgcgca ggtcaaacaa atctacaaga
     3781 caccacccat aaagtacttc ggcgggttca atttcagcca aatcctgccg gacccttcca
     3841 aacctagtaa gaggtcattc attgaggatc ttctgtttaa caaagttacg cttgcggacg
     3901 cgggattcat taagcagtat ggtgactgcc ttggagatat tgccgccagg gatttgatat
     3961 gtgcacagaa atttaacggc ctcaccgttc tgccgcctct gctcaccgat gagatgatag
     4021 cgcagtacac gagcgcactc ctggcaggta caattacaag cggatggaca ttcggtgcag
     4081 gagcagcgtt gcagataccc tttgctatgc agatggctta tcgatttaac gggattggcg
     4141 tcacgcagaa cgtcctttat gagaatcaga aattgattgc aaatcagttc aatagtgcta
     4201 tcggtaagat tcaggacagc ttgtttagta ccgctagcgc actgggaaag ttgcaggacg
     4261 tggtgaatca taatgcacaa gcactgaata ccttggttaa gcaattgagt agcaaattcg
     4321 gcgccatatc aagtgtactg aatgatatcc tgtcacggtt ggacaaggta gaagccgaag
     4381 ttcagattga ccgcttgatc accgggcgcc tccaaagtct gcagacctac gtcacacaac
     4441 aattgatcag agcagcagag ataagagcat ctgctaacct ggctgccact aagatgtctg
     4501 aatgtgtgct tgggcagtca aagagggtag atttctgcgg aaagggctac caccttatgt
     4561 ctttccctca gagcgctccg catggtgtgg tctttctcca tgtgacttat gtgcctgctc
     4621 aagagaagaa ctttacgacg gcgcccgcta tatgccatga tggtaaggcg cactttccaa
     4681 gggagggagt gttcgtgtcc aacggcactc actggtttgt cacccaacga aatttctacg
     4741 agcctcaaat tattaccacc gacaatacct ttgttagcgg taactgtgac gtcgtaattg
     4801 ggattgttaa taatacagtc tacgatcctc tgcagctgga actggactcc tttaaagagg
     4861 agctggacaa atatttcaag aaccacacat ctcctgacgt agatcttgga gacataagcg
     4921 gtataaatgc aagtgttgtt aacattcaga aagaaataga taggttgaac gaagttgcga
     4981 agaaccttaa cgagtcactg atagacctcc aagagcttgg gaagtacgag caatatatca
     5041 agtggccttg gtatatttgg ctcgggttca tagcaggact tatcgctata gtcatggtga
     5101 ctataatgct gtgctgcatg acaagctgct gcagctgtct caaaggctgt tgctcttgcg
     5161 gctcttgctg caaattcgat gaggacgatt ccgaacctgt cttgaagggt gtcaagctcc
     5221 attacacata actcgagggc ccagatctaa ttcaccccac cagtgcaggc tgcctatcag
     5281 aaagtggtgg ctggtgtggc taatgccctg gcccacaagt atcactaagc tcgctttctt
     5341 gctgtccaat ttctattaaa ggttcctttg ttccctaagt ccaactacta aactggggga
     5401 tattatgaag ggccttgagc atctggattc tgcctaataa aaaacattta ttttcattgc
     5461 aatgatgtat ttaaattatt tctgaatatt ttactaaaaa gggaatgtgg gaggtcagtg
     5521 catttaaaac ataaagaaat gaagagctag ttcaaacctt gggaaaatac actatatctt
     5581 aaactccatg aaagaaggtg aggctgcaaa cagctaatgc acattggcaa cagcccctga
     5641 tgcctatgcc ttattcatcc ctcagaaaag gattcaagta gaggcttgat ttggaggtta
     5701 aagttttgct atgctgtatt ttacattact tattgtttta gctgtcctca tgaatgtctt
     5761 ttcactaccc atttgcttat cctgcatctc tcagccttga ctccactcag ttctcttgct
     5821 tagagatacc acctttcccc tgaagtgttc cttccatgtt ttacggcgag atggtttctc
     5881 ctcgcctggc cactcagcct tagttgtctc tgttgtctta tagaggtcta cttgaagaag
     5941 gaaaaacagg gggcatggtt tgactgtcct gtgagccctt cttccctgcc tcccccactc
     6001 acagtgaccc ggaatccctc gacatggcag tctagatcat tcttgaagac gaaagggcct
     6061 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     6121 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6181 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6241 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6301 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6361 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6421 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6481 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6541 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6601 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6661 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6721 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6781 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6841 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6901 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6961 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7021 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7081 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7141 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7201 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7261 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7321 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7381 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7441 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7501 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7561 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7621 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7681 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7741 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7801 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7861 atggaaaaac gccagcaacg gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
     7921 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
     7981 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
     8041 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc
     8101 ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc cccgtgacga
     8161 tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc
     8221 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
     8281 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc ggggagcttt
     8341 ttgcaaaag
//
