LOCUS       Exported                8341 bp DNA     circular SYN 27-SEP-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8341
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="color: #------"
     CDS             1423..5223
                     /codon_start=1
                     /label=Lambda_Spike_cDNA
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNVIKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHNSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDA
                     VDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRF
                     ASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDE
                     VRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYQYRLFRKSNLKPFE
                     RDISTEIYQAGSTPCNGVEGFNCYSPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATV
                     CGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLE
                     ILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNV
                     FQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGA
                     ENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFC
                     TQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIE
                     DLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLNVLPPLLTDEMIAQYTSALL
                     AGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDS
                     LSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRL
                     ITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQS
                     APHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQI
                     ITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGIN
                     ASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTI
                     MLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     polyA_signal    5321..5715
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6135..6239
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6240..7100
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6240 .. 6308 / #ccffcc / signal sequence
                        2: 6309 .. 7100 / #ccffcc
                     Cleavage site after base 6308"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7271..7859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattccgcg ggcggccgca ccatgttcgt gttcctggta
     1441 ctccttcctt tggtgtcttc ccagtgtgta aatcttacca ctcggaccca gcttccaccc
     1501 gcctacacca acagtttcac acgcggcgtc tactatcctg acaaggtgtt taggagttca
     1561 gtcttgcact caactcaaga cttgttcctc cctttcttta gcaatgtgac gtggtttcat
     1621 gccattcatg tctccggcac aaacgttatc aagcgctttg ataatcctgt gctcccgttc
     1681 aacgatggag tgtacttcgc gtccacagag aagagcaata tcattcgagg ttggatcttc
     1741 ggaacgacac tcgactcaaa gacgcagtcc cttctcatcg tcaataatgc cacgaacgtg
     1801 gtcatcaaag tgtgcgagtt tcaattctgt aatgatccct tcctgggcgt ctattatcac
     1861 aagaacaaca aatcctggat ggagtccgaa tttagagtct actccagcgc caacaactgc
     1921 actttcgaat acgtatcaca gccattcttg atggaccttg aaggaaagca gggtaatttc
     1981 aagaacttga gggagttcgt attcaagaat atcgacgggt actttaagat ttatagcaaa
     2041 cacacaccca ttaatttggt gcgggatctt cctcagggat ttagtgctct tgagcctctc
     2101 gttgacctcc ctattggcat taacatcacc cgctttcaaa ccctgttggc cctgcataac
     2161 tctagttccg gatggaccgc aggtgccgcc gcatactatg tgggctacct tcagccaaga
     2221 acatttctgc tgaaatataa tgagaacggg accattacag atgcggtgga ttgtgcactc
     2281 gaccctctgt ctgagacgaa atgcaccctt aaaagcttca cggtggagaa aggcatttat
     2341 cagacttcta acttcagagt tcaacccacc gagtccattg tgcgattccc aaatattacg
     2401 aatttgtgcc catttggtga ggtcttcaat gctactcgat tcgcctcagt ttatgcatgg
     2461 aaccgaaaga gaatttccaa ttgtgtggcg gactactcag tattgtataa tagtgccagc
     2521 tttagcacat tcaaatgtta cggcgtgtct ccaacgaagc tgaacgatct ctgtttcaca
     2581 aacgtttatg cggattcctt cgtgattcgc ggcgatgagg tccgacagat tgcgcctggg
     2641 caaacgggta agatcgctga ttacaactat aagttgccgg acgatttcac aggatgtgtc
     2701 atagcttgga atagcaataa tttggacagt aaggttggcg gaaactacaa ttatcagtac
     2761 aggttgtttc gcaagtcaaa tttgaaacca tttgagagag atatatctac ggagatatat
     2821 caagccggct ctacaccatg taatggtgtg gagggcttca attgctactc tccactccag
     2881 tcatatggtt tccaacctac aaatggagta gggtatcaac cgtacagagt tgtggtcttg
     2941 agtttcgaat tgctccacgc tccagcaacg gtatgcggtc ctaagaaatc cacaaatctt
     3001 gtgaagaaca agtgcgtaaa tttcaacttc aatgggctga ctggtaccgg cgtgctgact
     3061 gagagtaaca agaagttctt gcctttccaa caattcgggc gggacatagc tgataccact
     3121 gacgccgtcc gcgaccctca gaccctggag attctggaca taactccttg ttctttcggt
     3181 ggcgtcagtg ttatcactcc cgggaccaac acctccaacc aagtcgcggt cctctatcaa
     3241 ggcgtcaact gtacagaagt accggtagcc atccatgcgg accaacttac accgacttgg
     3301 agggtttact ctacaggaag caatgtcttt caaacacgag ccgggtgtct gatcggagca
     3361 gaacacgtta acaacagcta cgaatgtgac ataccaatag gcgcagggat ttgtgcttca
     3421 tatcagacac agaccaatag cccgcggaga gcgagaagcg tagcaagcca aagcatcatc
     3481 gcgtacacga tgagcctcgg agcagagaac agcgtcgcgt atagcaataa ttcaatagct
     3541 atcccaacaa atttcactat ttcggtcacc actgagattc tgccggtctc catgaccaag
     3601 acatccgtcg attgtactat gtacatatgc ggcgacagca cggagtgcag taacttgctc
     3661 cttcagtacg gttccttctg tacgcagctt aaccgggcac tgacgggtat cgcggtagaa
     3721 caggacaaga acacacagga ggtcttcgcg caggtcaaac aaatctacaa gacaccaccc
     3781 ataaaggact tcggcgggtt caatttcagc caaatcctgc cggacccttc caaacctagt
     3841 aagaggtcat tcattgagga tcttctgttt aacaaagtta cgcttgcgga cgcgggattc
     3901 attaagcagt atggtgactg ccttggagat attgccgcca gagatctgat atgtgcacag
     3961 aaatttaacg gcctcaacgt tctgccgcct ctgctcaccg atgagatgat agcgcagtac
     4021 acgagcgcac tcctggcagg tacaattaca agcggatgga cattcggtgc aggagcagcg
     4081 ttgcagatac cctttgctat gcagatggct tatcgattta acgggattgg cgtcacgcag
     4141 aacgtccttt atgagaatca gaaattgatt gcaaatcagt tcaatagtgc tatcggtaag
     4201 attcaggaca gcttgagcag taccgctagc gcactgggaa agttgcagga cgtggtgaat
     4261 cagaatgcac aagcactgaa taccttggtt aagcaattga gtagcaattt cggcgccata
     4321 tcaagtgtac tgaatgatat cctgtcacgg ttggacaagg tagaagccga agttcagatt
     4381 gaccgcttga tcaccgggcg cctccaaagt ctgcagacct acgtcacaca acaattgatc
     4441 agagcagcag agataagagc atctgctaac ctggctgcca ctaagatgtc tgaatgtgtg
     4501 cttgggcagt caaagagggt agatttctgc ggaaagggct accaccttat gtctttccct
     4561 cagagcgctc cgcatggtgt ggtctttctc catgtgactt atgtgcctgc tcaagagaag
     4621 aactttacga cggcgcccgc tatatgccat gatggtaagg cgcactttcc aagggaggga
     4681 gtgttcgtgt ccaacggcac tcactggttt gtcacccaac gaaatttcta cgagcctcaa
     4741 attattacca ccgacaatac ctttgttagc ggtaactgtg acgtcgtaat tgggattgtt
     4801 aataatacag tctacgatcc tctgcagccg gaactggact cctttaaaga ggagctggac
     4861 aaatatttca agaaccacac atctcctgac gtagatcttg gagacataag cggtataaat
     4921 gcaagtgttg ttaacattca gaaagaaata gataggttga acgaagttgc gaagaacctt
     4981 aacgagtcac tgatagacct ccaagagctt gggaagtacg agcaatatat caagtggcct
     5041 tggtatattt ggctcgggtt catagcagga cttatcgcta tagtcatggt gactataatg
     5101 ctgtgctgca tgacaagctg ctgcagctgt ctcaaaggct gttgctcttg cggctcttgc
     5161 tgcaaattcg atgaggacga ttccgaacct gtcttgaagg gtgtcaagct ccattacaca
     5221 taactcgagg gcccagatct aattcacccc accagtgcag gctgcctatc agaaagtggt
     5281 ggctggtgtg gctaatgccc tggcccacaa gtatcactaa gctcgctttc ttgctgtcca
     5341 atttctatta aaggttcctt tgttccctaa gtccaactac taaactgggg gatattatga
     5401 agggccttga gcatctggat tctgcctaat aaaaaacatt tattttcatt gcaatgatgt
     5461 atttaaatta tttctgaata ttttactaaa aagggaatgt gggaggtcag tgcatttaaa
     5521 acataaagaa atgaagagct agttcaaacc ttgggaaaat acactatatc ttaaactcca
     5581 tgaaagaagg tgaggctgca aacagctaat gcacattggc aacagcccct gatgcctatg
     5641 ccttattcat ccctcagaaa aggattcaag tagaggcttg atttggaggt taaagttttg
     5701 ctatgctgta ttttacatta cttattgttt tagctgtcct catgaatgtc ttttcactac
     5761 ccatttgctt atcctgcatc tctcagcctt gactccactc agttctcttg cttagagata
     5821 ccacctttcc cctgaagtgt tccttccatg ttttacggcg agatggtttc tcctcgcctg
     5881 gccactcagc cttagttgtc tctgttgtct tatagaggtc tacttgaaga aggaaaaaca
     5941 gggggcatgg tttgactgtc ctgtgagccc ttcttccctg cctcccccac tcacagtgac
     6001 ccggaatccc tcgacatggc agtctagatc attcttgaag acgaaagggc ctcgtgatac
     6061 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6121 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6181 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6241 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6301 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6361 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6421 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6481 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6541 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6601 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6661 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6721 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6781 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6841 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6901 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6961 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7021 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7081 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7141 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7201 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7261 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7321 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7381 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7441 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7501 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7561 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7621 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7681 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7741 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7801 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7861 acgccagcaa cggatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt
     7921 ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct
     7981 tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg
     8041 ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc
     8101 catgccaacc cgttccatgt gctcgccgag gcggcataaa tccccgtgac gatcagcggt
     8161 ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg
     8221 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     8281 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcggggagct ttttgcaaaa
     8341 g
//
