LOCUS       Exported                8353 bp DNA     circular SYN 21-DEC-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2021 from SnapGene 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="color: #------"
     CDS             1423..5235
                     /label=SARS_CoV-2_Omicron_(B.1.1529)_Spike
                     /note="color: #800080; direction: RIGHT"
     polyA_signal    5333..5727
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6147..6251
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6252..7112
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6252 .. 6320 / #ccffcc / signal sequence
                        2: 6321 .. 7112 / #ccffcc
                     Cleavage site after base 6320"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7283..7871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattccgcg ggcggccgca ccatgttcgt gttcctggta
     1441 ctccttcctt tggtgtcttc ccagtgtgta aatcttacca ctcggaccca gcttccaccc
     1501 gcctacacca acagtttcac acgcggcgtc tactatcctg acaaggtgtt taggagttca
     1561 gtcttgcact caactcaaga cttgttcctc cctttcttta gcaatgtgac gtggtttcat
     1621 gtcatttccg gcacaaacgg aacgaagcgc tttgataatc ctgtgctccc gttcaacgat
     1681 ggagtgtact tcgcgtccat cgagaagagc aatatcattc gaggttggat cttcggaacg
     1741 acactcgact caaagacgca gtcccttctc atcgtcaata atgccacgaa cgtggtcatc
     1801 aaagtgtgcg agtttcaatt ctgtaatgat cccttcctgg atcacaagaa caacaaatcc
     1861 tggatggagt ccgaatttag agtctactcc agcgccaaca actgcacttt cgaatacgta
     1921 tcacagccat tcttgatgga ccttgaagga aagcagggta atttcaagaa cttgagggag
     1981 ttcgtattca agaatatcga cgggtacttt aagatttata gcaaacacac acccattatt
     2041 gtgcgggaac cagaggatct tcctcaggga tttagtgctc ttgagcctct cgttgacctc
     2101 cctattggca ttaacatcac ccgctttcaa accctgttgg ccctgcatcg gtcctacctg
     2161 acaccgggcg actcaagttc cggatggacc gcaggtgccg ccgcatacta tgtgggctac
     2221 cttcagccaa gaacatttct gctgaaatat aatgagaacg ggaccattac agatgcggtg
     2281 gattgtgcac tcgaccctct gtctgagacg aaatgcaccc ttaaaagctt cacggtggag
     2341 aaaggcattt atcagacttc taacttcaga gttcaaccca ccgagtccat tgtgcgattc
     2401 ccaaatatta cgaatttgtg cccatttgac gaggtcttca atgctactcg attcgcctca
     2461 gtttatgcat ggaaccgaaa gagaatttcc aattgtgtgg cggactactc agtattgtat
     2521 aatctcgccc cattttttac attcaaatgt tacggcgtgt ctccaacgaa gctgaacgat
     2581 ctctgtttca caaacgttta tgcggattcc ttcgtgattc gcggcgatga ggtccgacag
     2641 attgcgcctg ggcaaacggg taatatcgct gattacaact ataagttgcc ggacgatttc
     2701 acaggatgtg tcatagcttg gaatagcaat aagttggaca gtaaggttag tggaaactac
     2761 aattatttgt acaggttgtt tcgcaagtca aatttgaaac catttgagag agatatatct
     2821 acggagatat atcaagccgg caataagcca tgtaatggtg tggccggctt taactgctac
     2881 tttccactca agtcatattc attcagacct acatatggag tagggcacca accgtacaga
     2941 gttgtggtct tgagtttcga attgctccac gctccagcaa cggtatgcgg tcctaagaaa
     3001 tccacaaatc ttgtgaagaa caagtgcgta aatttcaact tcaatgggct gaagggtacc
     3061 ggcgtgctga ctgagagtaa caagaagttc ttgcctttcc aacaattcgg gcgggacata
     3121 gctgatacca ctgacgccgt ccgcgaccct cagaccctgg agattctgga cataactcct
     3181 tgttctttcg gtggcgtcag tgttatcact cccgggacca acacctccaa ccaagtcgcg
     3241 gtcctctatc aaggcgtcaa ctgtacggaa gtaccggtag ccatccatgc ggaccaactt
     3301 acaccgactt ggagggttta ctctacagga agcaatgtct ttcaaacacg agccgggtgt
     3361 ctgatcggag cagaatatgt taacaacagc tacgaatgtg acataccaat aggcgcaggg
     3421 atttgtgctt catatcagac acagaccaag agccaccgga gagcgagaag cgtagcaagc
     3481 caaagcatca tcgcgtacac gatgagcctc ggagcagaga acagcgtcgc gtatagcaat
     3541 aattcaatag ctatcccaac aaatttcact atttcggtca ccactgagat tctgccggtc
     3601 tccatgacca agacatccgt cgattgtact atgtacatat gcggcgacag cacggagtgc
     3661 agtaacttgc tccttcagta cggttccttc tgtacgcagc ttaaacgggc actgacgggt
     3721 atcgcggtag aacaggacaa gaacacacag gaggtcttcg cgcaggtcaa acaaatctac
     3781 aagacaccac ccataaagta tttcggcggg ttcaatttca gccaaatcct gccggaccct
     3841 tccaaaccta gtaagaggtc attcattgag gatcttctgt ttaacaaagt tacgcttgcg
     3901 gacgcgggat tcattaagca gtatggtgac tgccttggag atattgccgc cagggatttg
     3961 atatgtgcac agaaatttaa aggcctcacc gttctgccgc ctctgctcac cgatgagatg
     4021 atagcgcagt acacgagcgc actcctggca ggtacaatta caagcggatg gacattcggt
     4081 gcaggagcag cgttgcagat accctttgct atgcagatgg cttatcgatt taacgggatt
     4141 ggcgtcacgc agaacgtcct ttatgagaat cagaaattga ttgcaaatca gttcaatagt
     4201 gctatcggta agattcagga cagcttgagc agtaccgcta gcgcactggg aaagttgcag
     4261 gacgtggtga atcacaatgc acaagcactg aataccttgg ttaagcaatt gagtagcaaa
     4321 ttcggcgcca tatcaagtgt actgaatgac atcttttcac ggttggacaa ggtagaagcc
     4381 gaagttcaga ttgaccgctt gatcaccggg cgcctccaaa gtctgcagac ctacgtcaca
     4441 caacaattga tcagagcagc agagataaga gcatctgcta acctggctgc cactaagatg
     4501 tctgaatgtg tgcttgggca gtcaaagagg gtagatttct gcggaaaggg ctaccacctt
     4561 atgtctttcc ctcagagcgc tccgcatggt gtggtctttc tccatgtgac ttatgtgcct
     4621 gctcaagaga agaactttac gacggcgccc gctatatgcc atgatggtaa ggcgcacttt
     4681 ccaagggagg gagtgttcgt gtccaacggc actcactggt ttgtcaccca acgaaatttc
     4741 tacgagcctc aaattattac caccgacaat acctttgtta gcggtaactg tgacgtcgta
     4801 attgggattg ttaataatac agtctacgat cctctgcagc cggaactgga ctcctttaaa
     4861 gaggagctgg acaaatattt caagaaccac acatctcctg acgtagatct tggagacata
     4921 agcggtataa atgcaagtgt tgttaacatt cagaaagaaa tagataggtt gaacgaagtt
     4981 gcgaagaacc ttaacgagtc actgatagac ctccaagagc ttgggaagta cgagcaatat
     5041 atcaagtggc cttggtatat ttggctcggg ttcatagcag gacttatcgc tatagtcatg
     5101 gtgactataa tgctgtgctg catgacaagc tgctgcagct gtctcaaagg ctgttgctct
     5161 tgcggctctt gctgcaaatt cgatgaggac gattccgaac ctgtcttgaa gggtgtcaag
     5221 ctccattaca cataactcga gggcccagat ctaattcacc ccaccagtgc aggctgccta
     5281 tcagaaagtg gtggctggtg tggctaatgc cctggcccac aagtatcact aagctcgctt
     5341 tcttgctgtc caatttctat taaaggttcc tttgttccct aagtccaact actaaactgg
     5401 gggatattat gaagggcctt gagcatctgg attctgccta ataaaaaaca tttattttca
     5461 ttgcaatgat gtatttaaat tatttctgaa tattttacta aaaagggaat gtgggaggtc
     5521 agtgcattta aaacataaag aaatgaagag ctagttcaaa ccttgggaaa atacactata
     5581 tcttaaactc catgaaagaa ggtgaggctg caaacagcta atgcacattg gcaacagccc
     5641 ctgatgccta tgccttattc atccctcaga aaaggattca agtagaggct tgatttggag
     5701 gttaaagttt tgctatgctg tattttacat tacttattgt tttagctgtc ctcatgaatg
     5761 tcttttcact acccatttgc ttatcctgca tctctcagcc ttgactccac tcagttctct
     5821 tgcttagaga taccaccttt cccctgaagt gttccttcca tgttttacgg cgagatggtt
     5881 tctcctcgcc tggccactca gccttagttg tctctgttgt cttatagagg tctacttgaa
     5941 gaaggaaaaa cagggggcat ggtttgactg tcctgtgagc ccttcttccc tgcctccccc
     6001 actcacagtg acccggaatc cctcgacatg gcagtctaga tcattcttga agacgaaagg
     6061 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6121 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6181 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6241 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6301 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6361 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6421 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6481 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6541 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6601 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6661 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6721 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6781 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6841 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6901 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6961 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7021 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7081 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7141 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7201 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7321 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7381 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7441 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7501 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7561 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7621 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7681 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7741 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7801 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7861 gcctatggaa aaacgccagc aacggatgcg ccgcgtgcgg ctgctggaga tggcggacgc
     7921 gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt
     7981 ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
     8041 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
     8101 gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatccccgtg
     8161 acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc
     8221 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
     8281 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcggggag
     8341 ctttttgcaa aag
//
