LOCUS       Exported                8361 bp DNA     circular SYN 27-SEP-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="color: #------"
     CDS             1422..5243
                     /codon_start=1
                     /label=Wuhan-1_D614G_Spike_cDNA
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGE
                     VFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK
                     SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIA
                     YTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLL
                     LQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKP
                     SKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIA
                     QYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSA
                     IGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEA
                     EVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYH
                     LMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR
                     NFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDL
                     GDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLI
                     AIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     polyA_signal    5341..5735
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6155..6259
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6260..7120
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6260 .. 6328 / #ccffcc / signal sequence
                        2: 6329 .. 7120 / #ccffcc
                     Cleavage site after base 6328"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7291..7879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattccagt gcggccgcac catgttcgtg ttcctggtac
     1441 tccttccttt ggtgtcttcc cagtgtgtaa atcttaccac tcggacccag cttccacccg
     1501 cctacaccaa cagtttcaca cgcggcgtct actatcctga caaggtgttt aggagttcag
     1561 tcttgcactc aactcaagac ttgttcctcc ctttctttag caatgtgacg tggtttcatg
     1621 ccattcatgt ctccggcaca aacggaacga agcgctttga taatcctgtg ctcccgttca
     1681 acgatggagt gtacttcgcg tccacagaga agagcaatat cattcgaggt tggatcttcg
     1741 gaacgacact cgactcaaag acgcagtccc ttctcatcgt caataatgcc acgaacgtgg
     1801 tcatcaaagt gtgcgagttt caattctgta atgatccctt cctgggcgtc tattatcaca
     1861 agaacaacaa atcctggatg gagtccgaat ttagagtcta ctccagcgcc aacaactgca
     1921 ctttcgaata cgtatcacag ccattcttga tggaccttga aggaaagcag ggtaatttca
     1981 agaacttgag ggagttcgta ttcaagaata tcgacgggta ctttaagatt tatagcaaac
     2041 acacacccat taatttggtg cgggatcttc ctcagggatt tagtgctctt gagcctctcg
     2101 ttgacctccc tattggcatt aacatcaccc gctttcaaac cctgttggcc ctgcatcggt
     2161 cctacctgac accgggcgac tcaagttccg gatggaccgc aggtgccgcc gcatactatg
     2221 tgggctacct tcagccaaga acatttctgc tgaaatataa tgagaacggg accattacag
     2281 atgcggtgga ttgtgcactc gaccctctgt ctgagacgaa atgcaccctt aaaagcttca
     2341 cggtggagaa aggcatttat cagacttcta acttcagagt tcaacccacc gagtccattg
     2401 tgcgattccc aaatattacg aatttgtgcc catttggtga ggtcttcaat gctactcgat
     2461 tcgcctcagt ttatgcatgg aaccgaaaga gaatttccaa ttgtgtggcg gactactcag
     2521 tattgtataa tagtgccagc tttagcacat tcaaatgtta cggcgtgtct ccaacgaagc
     2581 tgaacgatct ctgtttcaca aacgtttatg cggattcctt cgtgattcgc ggcgatgagg
     2641 tccgacagat tgcgcctggg caaacgggta agatcgctga ttacaactat aagttgccgg
     2701 acgatttcac aggatgtgtc atagcttgga atagcaataa tttggacagt aaggttggcg
     2761 gaaactacaa ttatttgtac aggttgtttc gcaagtcaaa tttgaaacca tttgagagag
     2821 atatatctac ggagatatat caagccggct ctacaccatg taatggtgtg gagggcttta
     2881 actgctactt tccactccag tcatatggtt tccaacctac aaatggagta gggtatcaac
     2941 cgtacagagt tgtggtcttg agtttcgaat tgctccacgc tccagcaacg gtatgcggtc
     3001 ctaagaaatc cacaaatctt gtgaagaaca agtgcgtaaa tttcaacttc aatgggctga
     3061 ctggtaccgg cgtgctgact gagagtaaca agaagttctt gcctttccaa caattcgggc
     3121 gggacatagc tgataccact gacgccgtcc gcgaccctca gaccctggag attctggaca
     3181 taactccttg ttctttcggt ggcgtcagtg ttatcactcc cgggaccaac acctccaacc
     3241 aagtcgcggt cctctatcaa ggcgtcaact gtacagaagt accggtagcc atccatgcgg
     3301 accaacttac accgacttgg agggtttact ctacaggaag caatgtcttt caaacacgag
     3361 ccgggtgtct gatcggagca gaacacgtta acaacagcta cgaatgtgac ataccaatag
     3421 gcgcagggat ttgtgcttca tatcagacac agaccaatag cccgcggaga gcgagaagcg
     3481 tagcaagcca aagcatcatc gcgtacacga tgagcctcgg agcagagaac agcgtcgcgt
     3541 atagcaataa ttcaatagct atcccaacaa atttcactat ttcggtcacc actgagattc
     3601 tgccggtctc catgaccaag acatccgtcg attgtactat gtacatatgc ggcgacagca
     3661 cggagtgcag taacttgctc cttcagtacg gttccttctg tacgcagctt aaccgggcac
     3721 tgacgggtat cgcggtagaa caggacaaga acacacagga ggtcttcgcg caggtcaaac
     3781 aaatctacaa gacaccaccc ataaaggact tcggcgggtt caatttcagc caaatcctgc
     3841 cggacccttc caaacctagt aagaggtcat tcattgagga tcttctgttt aacaaagtta
     3901 cgcttgcgga cgcgggattc attaagcagt atggtgactg ccttggagat attgccgcca
     3961 gggatttgat atgtgcacag aaatttaacg gcctcaccgt tctgccgcct ctgctcaccg
     4021 atgagatgat agcgcagtac acgagcgcac tcctggcagg tacaattaca agcggatgga
     4081 cattcggtgc aggagcagcg ttgcagatac cctttgctat gcagatggct tatcgattta
     4141 acgggattgg cgtcacgcag aacgtccttt atgagaatca gaaattgatt gcaaatcagt
     4201 tcaatagtgc tatcggtaag attcaggaca gcttgagcag taccgctagc gcactgggaa
     4261 agttgcagga cgtggtgaat cagaatgcac aagcactgaa taccttggtt aagcaattga
     4321 gtagcaattt cggcgccata tcaagtgtac tgaatgatat cctgtcacgg ttggacaagg
     4381 tagaagccga agttcagatt gaccgcttga tcaccgggcg cctccaaagt ctgcagacct
     4441 acgtcacaca acaattgatc agagcagcag agataagagc atctgctaac ctggctgcca
     4501 ctaagatgtc tgaatgtgtg cttgggcagt caaagagggt agatttctgc ggaaagggct
     4561 accaccttat gtctttccct cagagcgctc cgcatggtgt ggtctttctc catgtgactt
     4621 atgtgcctgc tcaagagaag aactttacga cggcgcccgc tatatgccat gatggtaagg
     4681 cgcactttcc aagggaggga gtgttcgtgt ccaacggcac tcactggttt gtcacccaac
     4741 gaaatttcta cgagcctcaa attattacca ccgacaatac ctttgttagc ggtaactgtg
     4801 acgtcgtaat tgggattgtt aataatacag tctacgatcc tctgcagccg gaactggact
     4861 cctttaaaga ggagctggac aaatatttca agaaccacac atctcctgac gtagatcttg
     4921 gagacataag cggtataaat gcaagtgttg ttaacattca gaaagaaata gataggttga
     4981 acgaagttgc gaagaacctt aacgagtcac tgatagacct ccaagagctt gggaagtacg
     5041 agcaatatat caagtggcct tggtatattt ggctcgggtt catagcagga cttatcgcta
     5101 tagtcatggt gactataatg ctgtgctgca tgacaagctg ctgcagctgt ctcaaaggct
     5161 gttgctcttg cggctcttgc tgcaaattcg atgaggacga ttccgaacct gtcttgaagg
     5221 gtgtcaagct ccattacaca taactcgagg gcccagatct aattcacccc accagtgcag
     5281 gctgcctatc agaaagtggt ggctggtgtg gctaatgccc tggcccacaa gtatcactaa
     5341 gctcgctttc ttgctgtcca atttctatta aaggttcctt tgttccctaa gtccaactac
     5401 taaactgggg gatattatga agggccttga gcatctggat tctgcctaat aaaaaacatt
     5461 tattttcatt gcaatgatgt atttaaatta tttctgaata ttttactaaa aagggaatgt
     5521 gggaggtcag tgcatttaaa acataaagaa atgaagagct agttcaaacc ttgggaaaat
     5581 acactatatc ttaaactcca tgaaagaagg tgaggctgca aacagctaat gcacattggc
     5641 aacagcccct gatgcctatg ccttattcat ccctcagaaa aggattcaag tagaggcttg
     5701 atttggaggt taaagttttg ctatgctgta ttttacatta cttattgttt tagctgtcct
     5761 catgaatgtc ttttcactac ccatttgctt atcctgcatc tctcagcctt gactccactc
     5821 agttctcttg cttagagata ccacctttcc cctgaagtgt tccttccatg ttttacggcg
     5881 agatggtttc tcctcgcctg gccactcagc cttagttgtc tctgttgtct tatagaggtc
     5941 tacttgaaga aggaaaaaca gggggcatgg tttgactgtc ctgtgagccc ttcttccctg
     6001 cctcccccac tcacagtgac ccggaatccc tcgacatggc agtctagatc attcttgaag
     6061 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     6121 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6181 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6241 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6301 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6361 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6421 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6481 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6541 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6601 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6661 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6721 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6781 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6841 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6901 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6961 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7021 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7081 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7141 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7201 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7261 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7321 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7381 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7441 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7501 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7561 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7621 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7681 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7741 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7801 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7861 ggggcggagc ctatggaaaa acgccagcaa cggatgcgcc gcgtgcggct gctggagatg
     7921 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     7981 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca
     8041 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     8101 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     8161 tccccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc
     8221 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     8281 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     8341 tcggggagct ttttgcaaaa g
//
