LOCUS       HDM_XBB.1.5_Spik        8350 bp DNA     circular SYN 08-MAY-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported May 8, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          877..1352
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
                     /note="color: #------"
     CDS             1423..5232
                     /codon_start=1
                     /label=SARS_CoV-2_XBB.1.5_Spike_cDNA
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNLITRTQSYTNSFTRGVYYPDKVFRSSVL
                     HSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPALPFNDGVYFASTEKSNIIRGWIFG
                     TTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLDVYQKNNKSWMESEFRVYSSANNCT
                     FEYVSQPFLMDLEGKEGNFKNLREFVFKNIDGYFKIYSKHTPINLERDLPQGFSALEPL
                     VDLPIGINITRFQTLLALHRSYLTPVDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTI
                     TDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFHEVFNA
                     TTFASVYAWNRKRISNCVADYSVIYNFAPFFAFKCYGVSPTKLNDLCFTNVYADSFVIR
                     GNEVSQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNKLDSKPSGNYNYLYRLFRKSKLK
                     PFERDISTEIYQAGNKPCNGVAGPNCYSPLQSYGFRPTYGVGHQPYRVVVLSFELLHAP
                     ATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQ
                     TLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTG
                     SNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTKSHRRARSVASQSIIAYTMS
                     LGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYG
                     SFCTQLKRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKYFGGFNFSQILPDPSKPSKRS
                     FIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTS
                     ALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKI
                     QDSLSSTASALGKLQDVVNHNAQALNTLVKQLSSKFGAISSVLNDILSRLDKVEAEVQI
                     DRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSF
                     PQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYE
                     PQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDIS
                     GINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVM
                     VTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     polyA_signal    5330..5724
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6144..6248
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6249..7109
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6249 .. 6317 / #ccffcc / signal sequence
                        2: 6318 .. 7109 / #ccffcc
                     Cleavage site after base 6317"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7280..7868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
       61 tcggcctctg cataaataaa aaaaattagt cagccatgag cttggcccat tgcatacgtt
      121 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      781 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      841 cagcctcccc tcgaagctga tcctgagaac ttcagggtga gtctatggga cccttgatgt
      901 tttctttccc cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg
      961 gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt
     1021 cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca
     1081 gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata
     1141 atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt
     1201 aactgatgta agaggtttca tattgctaat agcagctaca atccagctac cattctgctt
     1261 ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa
     1321 tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc
     1381 tggcccatca ctttggcaaa gaattcaatt ggcggccgca ccatgttcgt gttcctggta
     1441 ctccttcctt tggtgtcttc ccagtgtgta aatcttatca ctcggaccca gtcttacacc
     1501 aacagtttca cacgcggcgt ctactatcct gacaaggtgt ttaggagttc agtcttgcac
     1561 tcaactcaag acttgttcct ccctttcttt agcaatgtga cgtggtttca tgccattcat
     1621 gtctccggca caaacggaac gaagcgcttt gataatcctg ccctcccgtt caacgatgga
     1681 gtgtacttcg cgtccacaga gaagagcaat atcattcgag gttggatctt cggaacgaca
     1741 ctcgactcaa agacgcagtc ccttctcatc gtcaataatg ccacgaacgt ggtcatcaaa
     1801 gtgtgcgagt ttcaattctg taatgatccc ttcctggacg tctatcagaa gaacaacaaa
     1861 tcctggatgg agtccgaatt tagagtctac tccagcgcca acaactgcac tttcgaatac
     1921 gtatcacagc cattcttgat ggaccttgaa ggaaaggagg gtaatttcaa gaacttgagg
     1981 gagttcgtat tcaagaatat cgacgggtac tttaagattt atagcaaaca cacacccatt
     2041 aatttggagc gggatcttcc tcagggattt agtgctcttg agcctctcgt tgacctccct
     2101 attggcatta acatcacccg ctttcaaacc ctgttggccc tgcatcggtc ctacctgaca
     2161 cccgtggact caagttccgg atggaccgca ggtgccgccg catactatgt gggctacctt
     2221 cagccaagaa catttctgct gaaatataat gagaacggga ccattacaga tgcggtggat
     2281 tgtgcactcg accctctgtc tgagacgaaa tgcaccctta aaagcttcac ggtggagaaa
     2341 ggcatttatc agacttctaa cttcagagtt caacccaccg agtccattgt gcgattccca
     2401 aatattacga atttgtgccc atttcacgag gtcttcaatg ctactacgtt cgcctcagtt
     2461 tatgcatgga accgaaagag aatttccaat tgtgtggcgg actactcagt aatttataat
     2521 tttgccccct tttttgcctt caaatgttac ggcgtgtctc caacgaagct gaacgatctc
     2581 tgtttcacaa acgtttatgc ggattccttc gtgattcgcg gcaacgaggt cagccagatt
     2641 gcgcctgggc aaacgggtaa tatcgctgat tacaactata agttgccgga cgatttcaca
     2701 ggatgtgtca tagcttggaa tagcaataaa ttggacagta agccctctgg aaactacaat
     2761 tatttgtaca ggttgtttcg caagtcaaaa ttgaaaccat ttgagagaga tatatctacg
     2821 gagatatatc aagccggcaa caaaccatgt aatggtgtgg ccggccccaa ttgctactct
     2881 ccactccagt catatggttt caggcctaca tatggagtag ggcaccaacc gtacagagtt
     2941 gtggtcttga gtttcgaatt gctccacgct ccagcaacgg tatgcggtcc taagaaatcc
     3001 acaaatcttg tgaagaacaa gtgcgtaaat ttcaacttca atgggctgac tggtaccggc
     3061 gtgctgactg agagtaacaa gaagttcttg cctttccaac aattcgggcg ggacatagct
     3121 gataccactg acgccgtccg cgaccctcag accctggaga ttctggacat aactccttgt
     3181 tctttcggtg gcgtcagtgt tatcactccc gggaccaaca cctccaacca agtcgcggtc
     3241 ctctatcaag gcgtcaactg tacggaagta ccggtagcca tccatgcgga ccaacttaca
     3301 ccgacttgga gggtttactc tacaggaagc aatgtctttc aaacacgagc cgggtgtctg
     3361 atcggagcag aatatgttaa caacagctac gaatgtgaca taccaatagg cgcagggatt
     3421 tgtgcttcat atcagacaca gaccaaaagc caccggagag cgagaagcgt agcaagccaa
     3481 agcatcatcg cgtacacgat gagcctcgga gcagagaaca gcgtcgcgta tagcaataat
     3541 tcaatagcta tcccaacaaa tttcactatt tcggtcacca ctgagattct gccggtctcc
     3601 atgaccaaga catccgtcga ttgtactatg tacatatgcg gcgacagcac ggagtgcagt
     3661 aacttgctcc ttcagtacgg ttccttctgt acgcagctta aacgggcact gacgggtatc
     3721 gcggtagaac aggacaagaa cacacaggag gtcttcgcgc aggtcaaaca aatctacaag
     3781 acaccaccca taaagtattt cggcgggttc aatttcagcc aaatcctgcc ggacccttcc
     3841 aaacctagta agaggtcatt cattgaggat cttctgttta acaaagttac gcttgcggac
     3901 gcgggattca ttaagcagta tggtgactgc cttggagata ttgccgccag ggatttgata
     3961 tgtgcacaga aatttaacgg cctcaccgtt ctgccgcctc tgctcaccga tgagatgata
     4021 gcgcagtaca cgagcgcact cctggcaggt acaattacaa gcggatggac attcggtgca
     4081 ggagcagcgt tgcagatacc ctttgctatg cagatggctt atcgatttaa cgggattggc
     4141 gtcacgcaga acgtccttta tgagaatcag aaattgattg caaatcagtt caatagtgct
     4201 atcggtaaga ttcaggacag cttgagcagt accgctagcg cactgggaaa gttgcaggac
     4261 gtggtgaatc acaatgcaca agcactgaat accttggtta agcaattgag tagcaagttc
     4321 ggcgccatat caagtgtact gaatgatatc ctgtcacggt tggacaaggt agaagccgaa
     4381 gttcagattg accgcttgat caccgggcgc ctccaaagtc tgcagaccta cgtcacacaa
     4441 caattgatca gagcagcaga gataagagca tctgctaacc tggctgccac taagatgtct
     4501 gaatgtgtgc ttgggcagtc aaagagggta gatttctgcg gaaagggcta ccaccttatg
     4561 tctttccctc agagcgctcc gcatggtgtg gtctttctcc atgtgactta tgtgcctgct
     4621 caagagaaga actttacgac ggcgcccgct atatgccatg atggtaaggc gcactttcca
     4681 agggagggag tgttcgtgtc caacggcact cactggtttg tcacccaacg aaatttctac
     4741 gagcctcaaa ttattaccac cgacaatacc tttgttagcg gtaactgtga cgtcgtaatt
     4801 gggattgtta ataatacagt ctacgatcct ctgcagccgg aactggactc ctttaaagag
     4861 gagctggaca aatatttcaa gaaccacaca tctcctgacg tagatcttgg agacataagc
     4921 ggtataaatg caagtgttgt taacattcag aaagaaatag ataggttgaa cgaagttgcg
     4981 aagaacctta acgagtcact gatagacctc caagagcttg ggaagtacga gcaatatatc
     5041 aagtggcctt ggtatatttg gctcgggttc atagcaggac ttatcgctat agtcatggtg
     5101 actataatgc tgtgctgcat gacaagctgc tgcagctgtc tcaaaggctg ttgctcttgc
     5161 ggctcttgct gcaaattcga tgaggacgat tccgaacctg tcttgaaggg tgtcaagctc
     5221 cattacacat aactcgaggg cccagatcta attcacccca ccagtgcagg ctgcctatca
     5281 gaaagtggtg gctggtgtgg ctaatgccct ggcccacaag tatcactaag ctcgctttct
     5341 tgctgtccaa tttctattaa aggttccttt gttccctaag tccaactact aaactggggg
     5401 atattatgaa gggccttgag catctggatt ctgcctaata aaaaacattt attttcattg
     5461 caatgatgta tttaaattat ttctgaatat tttactaaaa agggaatgtg ggaggtcagt
     5521 gcatttaaaa cataaagaaa tgaagagcta gttcaaacct tgggaaaata cactatatct
     5581 taaactccat gaaagaaggt gaggctgcaa acagctaatg cacattggca acagcccctg
     5641 atgcctatgc cttattcatc cctcagaaaa ggattcaagt agaggcttga tttggaggtt
     5701 aaagttttgc tatgctgtat tttacattac ttattgtttt agctgtcctc atgaatgtct
     5761 tttcactacc catttgctta tcctgcatct ctcagccttg actccactca gttctcttgc
     5821 ttagagatac cacctttccc ctgaagtgtt ccttccatgt tttacggcga gatggtttct
     5881 cctcgcctgg ccactcagcc ttagttgtct ctgttgtctt atagaggtct acttgaagaa
     5941 ggaaaaacag ggggcatggt ttgactgtcc tgtgagccct tcttccctgc ctcccccact
     6001 cacagtgacc cggaatccct cgacatggca gtctagatca ttcttgaaga cgaaagggcc
     6061 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6241 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6301 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6361 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6421 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6481 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6541 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6601 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6661 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6721 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6781 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6841 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6901 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6961 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7021 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7081 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7141 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7201 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7261 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7321 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7381 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7441 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7501 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7561 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7621 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7681 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7741 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7801 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7861 tatggaaaaa cgccagcaac ggatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     7921 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     7981 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     8041 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     8101 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat ccccgtgacg
     8161 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     8221 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     8281 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cggggagctt
     8341 tttgcaaaag
//
