LOCUS       pSTVH7_C-BirA_pD       10520 bp    DNA     circular     03-AUG-2018
DEFINITION  .
ACCESSION   urn.local...1a-9g2vho4
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com Cat.
            No. 632158,632162,632157,632163.
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label="5'\LTR"
     misc_binding    636..653
                     /label="PBS"
     misc_feature    685..822
                     /label="Packaging\signal"
     misc_binding    1303..1536
                     /label="RRE"
     misc_feature    2028..2151
                     /label="cPPT/CTS"
     CDS             complement(2455..2481)
                     /ApEinfo_fwdcolor="#006cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Flag"
     CDS             complement(2512..3474)
                     /ApEinfo_fwdcolor="#006cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BirA"
     attR2           complement(3485..3609)
                     /created_by="User"
                     /modified_by="User"
                     /label="ATTR2"
     CDS             complement(3650..3955)
                     /ApEinfo_fwdcolor="#00ff28"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ccdb"
     CDS             complement(4297..4956)
                     /ApEinfo_fwdcolor="#00ff28"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Chlorm"
     attR1           complement(5065..5189)
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="ATTR1"
     promoter        complement(5219..5534)
                     /created_by="User"
                     /label="TRE"
     promoter        5579..5805
                     /created_by="User"
                     /label="EF1a-core"
     CDS             5826..6425
                     /label="Puro(R)"
     WPRE            6442..7033
                     /modified_by="User"
                     /label="WPRE"
     LTR             7236..7872
                     /label="3'\LTR"
     rep_origin      complement(8341..9014)
                     /label="pUC\origin"
     CDS             complement(9159..10155)
                     /label="Amp(R)"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtg catgcctgct
     2221 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     2281 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
     2341 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
     2401 agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctctagact cgagttattt
     2461 atcgtcatcg tctttgtagt ctggcgcgcc gccgccgggg ccagatcccc ccttctcggc
     2521 gctccgcagg ctgatctcgc cgcccatcca gggcttgatg atgccgtcct gctccagcag
     2581 cagggcgccc tgcttgtcga tgccccggct gatgccgaag atctccttgt cgccgatgat
     2641 cagcttcacg ggccggttga tgaagttgtc cagcttctcc caccggctca ggtagggggc
     2701 caggccctcc tgctcgaaca gctccagggc ggcccgcagc tcccggatca gcatggcggc
     2761 cagggtgttc cggtccaggt tgatgccggc ctcctgcagg gtgatccagc cctggttcac
     2821 cacgctctcc tccacccgcc gcatggccat gttgatgccg gcgccgatca cgatctgggc
     2881 ggcgtcgccg gtcttgccgg tcagctccac caggatgccg gccagcttcc ggtcctgcag
     2941 gtacaggtcg ttgggccact tcacccgcac cttgtcggcg cccagcttcc gcagcacctc
     3001 ggccatcacg atgccgatca ccaggctcag gccgatggcg gcggcggggc cctgctccag
     3061 ccgccagaac atgctcaggt acaggttggc gccgaagggg ctgaaccact tccggccccg
     3121 gccgccccgg ccggcctgct ggtactcggc gatgcaggcg tcgccgctct tcagctcgcc
     3181 gatccggtcc agcaggtact ggttggtgct gtcgatcacg ggcagcacgg ccacgctgcc
     3241 gccgtccagc tggcccagga tctgcttggc gttcagcagc tggatgggct cgggcaggct
     3301 gtagcccttg ccgggcacgg tgaacacgtc cacgccccag tcccgcaggg tctggatgtg
     3361 cttgttgatg gcggcccggc tcatgcccag ggtctcgccc agctgctcgc cgctgtggaa
     3421 ctcgccgttg gccagcaggg cgatcagctt caggggcacg gtgttgtcct tcattctaga
     3481 atcaaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat
     3541 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     3601 gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
     3661 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
     3721 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
     3781 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
     3841 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
     3901 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
     3961 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
     4021 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
     4081 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
     4141 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
     4201 tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata
     4261 ctgttatctg gcttttagta agccggatcc agatctttac gccccgccct gccactcatc
     4321 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     4381 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     4441 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     4501 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     4561 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     4621 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     4681 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
     4741 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     4801 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     4861 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     4921 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     4981 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
     5041 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
     5101 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     5161 tctcgttcag cttttttgta caaacttgtc taaagcttag gccgcggatc cttctccagg
     5221 cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcctac
     5281 ctcgacatac gttctctatc actgataggg agtaaactcg acatacgttc tctatcactg
     5341 atagggataa actcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt
     5401 ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata gggagtaaac
     5461 tcgacatcgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
     5521 agggagtaaa ctctagtgaa gacgaaaggg ccagacgcgg ccgctcaacg cgtgaattcg
     5581 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     5641 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     5701 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     5761 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggaactg cagcccaagc
     5821 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     5881 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     5941 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6001 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6061 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6121 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     6181 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     6241 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6301 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     6361 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6421 cctgacgggc gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     6481 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6541 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6601 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6661 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6721 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6781 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     6841 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6901 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6961 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7021 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     7081 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     7141 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     7201 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     7261 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     7321 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     7381 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     7441 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     7501 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     7561 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     7621 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     7681 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7741 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7801 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7861 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7921 tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag
     7981 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8041 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8101 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8161 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8221 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8821 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9061 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9121 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9181 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9241 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9301 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9361 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9421 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9481 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9541 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9601 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9661 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9721 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9781 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9841 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9901 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9961 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10021 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10081 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10141 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
    10201 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10261 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    10321 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    10381 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    10441 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata
    10501 tgtactacaa acttagtagt
//
