LOCUS       pSTVH7_N-BirA_pD       10551 bp    DNA     circular     03-AUG-2018
DEFINITION  .
ACCESSION   urn.local...h-9g2vfjh
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com Cat.
            No. 632158,632162,632157,632163.
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label="5'\LTR"
     misc_binding    636..653
                     /label="PBS"
     misc_feature    685..822
                     /label="Packaging\signal"
     misc_binding    1303..1536
                     /label="RRE"
     misc_feature    2028..2151
                     /label="cPPT/CTS"
     attR2           complement(2459..2583)
                     /created_by="User"
                     /modified_by="User"
                     /label="ATTR2"
     CDS             complement(2624..2929)
                     /ApEinfo_fwdcolor="#00dd14"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ccdB"
     CDS             complement(3271..3930)
                     /ApEinfo_fwdcolor="#00dd14"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Chlorm"
     attR1           complement(4039..4163)
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="ATTR1"
     CDS             complement(4176..4199)
                     /ApEinfo_fwdcolor="#006ddd"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="FLAG"
     CDS             complement(4230..5192)
                     /ApEinfo_fwdcolor="#2474dd"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BirA"
     promoter        complement(5250..5565)
                     /created_by="User"
                     /label="TRE"
     promoter        5610..5836
                     /created_by="User"
                     /label="EF1a-core"
     CDS             5857..6456
                     /label="Puro(R)"
     WPRE            6473..7064
                     /modified_by="User"
                     /label="WPRE"
     LTR             7267..7903
                     /label="3'\LTR"
     rep_origin      complement(8372..9045)
                     /label="pUC\origin"
     CDS             complement(9190..10186)
                     /label="Amp(R)"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtg catgcctgct
     2221 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     2281 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
     2341 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
     2401 agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctctagact cgagtcacac
     2461 cactttgtac aagaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     2521 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     2581 atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
     2641 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
     2701 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
     2761 cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg
     2821 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
     2881 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc
     2941 ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct
     3001 tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt
     3061 gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc
     3121 tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt
     3181 atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta
     3241 tctggctttt agtaagccgg atccagatct ttacgccccg ccctgccact catcgcagta
     3301 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     3361 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     3421 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     3481 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     3541 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     3601 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     3661 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
     3721 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     3781 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     3841 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     3901 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     3961 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
     4021 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     4081 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     4141 tcagcttttt tgtacaaact tgtgggcgcg cctagtttat cgtcatcgtc tttgtagtct
     4201 ggcgcgccgc cgccggggcc agatcccccc ttctcggcgc tccgcaggct gatctcgccg
     4261 cccatccagg gcttgatgat gccgtcctgc tccagcagca gggcgccctg cttgtcgatg
     4321 ccccggctga tgccgaagat ctccttgtcg ccgatgatca gcttcacggg ccggttgatg
     4381 aagttgtcca gcttctccca ccggctcagg tagggggcca ggccctcctg ctcgaacagc
     4441 tccagggcgg cccgcagctc ccggatcagc atggcggcca gggtgttccg gtccaggttg
     4501 atgccggcct cctgcagggt gatccagccc tggttcacca cgctctcctc cacccgccgc
     4561 atggccatgt tgatgccggc gccgatcacg atctgggcgg cgtcgccggt cttgccggtc
     4621 agctccacca ggatgccggc cagcttccgg tcctgcaggt acaggtcgtt gggccacttc
     4681 acccgcacct tgtcggcgcc cagcttccgc agcacctcgg ccatcacgat gccgatcacc
     4741 aggctcaggc cgatggcggc ggcggggccc tgctccagcc gccagaacat gctcaggtac
     4801 aggttggcgc cgaaggggct gaaccacttc cggccccggc cgccccggcc ggcctgctgg
     4861 tactcggcga tgcaggcgtc gccgctcttc agctcgccga tccggtccag caggtactgg
     4921 ttggtgctgt cgatcacggg cagcacggcc acgctgccgc cgtccagctg gcccaggatc
     4981 tgcttggcgt tcagcagctg gatgggctcg ggcaggctgt agcccttgcc gggcacggtg
     5041 aacacgtcca cgccccagtc ccgcagggtc tggatgtgct tgttgatggc ggcccggctc
     5101 atgcccaggg tctcgcccag ctgctcgccg ctgtggaact cgccgttggc cagcagggcg
     5161 atcagcttca ggggcacggt gttgtccttc atggtggcgc tagctggatc cgagctcggt
     5221 accaagctta ggccgcggat ccttctccag gcgatctgac ggttcactaa acgagctctg
     5281 cttatatagg cctcccaccg tacacgccta cctcgacata cgttctctat cactgatagg
     5341 gagtaaactc gacatacgtt ctctatcact gatagggata aactcgacat acgttctcta
     5401 tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag taaactcgac
     5461 atacgttctc tatcactgat agggagtaaa ctcgacatcg ttctctatca ctgataggga
     5521 gtaaactcga catacgttct ctatcactga tagggagtaa actctagtga agacgaaagg
     5581 gccagacgcg gccgctcaac gcgtgaattc ggtgcccgtc agtgggcaga gcgcacatcg
     5641 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     5701 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5761 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5821 gccgccagaa cacaggaact gcagcccaag cttaccatga ccgagtacaa gcccacggtg
     5881 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     5941 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6001 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6061 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6121 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6181 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6241 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6301 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6361 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6421 cgcacctggt gcatgacccg caagcccggt gcctgacggg cgcgtctgga acaatcaacc
     6481 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6541 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6601 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6661 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6721 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6781 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6841 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     6901 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6961 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7021 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     7081 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     7141 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     7201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7261 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     7321 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     7381 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     7441 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     7501 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     7561 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     7621 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     7681 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     7741 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7801 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7861 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7921 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     7981 ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     8041 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8101 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8161 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8341 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8941 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9001 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9061 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    10201 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10261 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10321 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    10381 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    10441 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10501 tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
