LOCUS       pSTVH8_C-BirA_pD       10543 bp    DNA     circular     29-AUG-2018
DEFINITION  .
ACCESSION   urn.local...70-9fz08y9
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com Cat.
            No. 632158,632162,632157,632163.
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label="5'\LTR"
     misc_binding    636..653
                     /label="PBS"
     misc_feature    685..822
                     /label="Packaging\signal"
     misc_binding    1303..1536
                     /label="RRE"
     misc_feature    2028..2151
                     /label="cPPT/CTS"
     CDS             complement(2455..2481)
                     /ApEinfo_fwdcolor="#006cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Flag"
     CDS             complement(2512..3474)
                     /ApEinfo_fwdcolor="#006cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BirA"
     attR2           complement(3485..3609)
                     /created_by="User"
                     /modified_by="User"
                     /label="ATTR2"
     CDS             complement(3650..3955)
                     /ApEinfo_fwdcolor="#00ff28"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ccdb"
     CDS             complement(4297..4956)
                     /ApEinfo_fwdcolor="#00ff28"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Chlorm"
     attR1           complement(5065..5189)
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="ATTR1"
     promoter        complement(5219..5534)
                     /created_by="User"
                     /label="TRE"
     promoter        5579..5805
                     /created_by="User"
                     /label="EF1a-core"
     CDS             5822..6568
                     /ApEinfo_fwdcolor="#ff9562"
                     /ApEinfo_revcolor="#ff8040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="rtTA"
     WPRE            6589..7164
                     /ApEinfo_fwdcolor="#008cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="WPRE"
     LTR             7259..7895
                     /label="3'\LTR"
     rep_origin      complement(8364..9037)
                     /label="pUC\origin"
     CDS             complement(9182..10178)
                     /label="Amp(R)"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtg catgcctgct
     2221 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     2281 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
     2341 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
     2401 agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctctagact cgagttattt
     2461 atcgtcatcg tctttgtagt ctggcgcgcc gccgccgggg ccagatcccc ccttctcggc
     2521 gctccgcagg ctgatctcgc cgcccatcca gggcttgatg atgccgtcct gctccagcag
     2581 cagggcgccc tgcttgtcga tgccccggct gatgccgaag atctccttgt cgccgatgat
     2641 cagcttcacg ggccggttga tgaagttgtc cagcttctcc caccggctca ggtagggggc
     2701 caggccctcc tgctcgaaca gctccagggc ggcccgcagc tcccggatca gcatggcggc
     2761 cagggtgttc cggtccaggt tgatgccggc ctcctgcagg gtgatccagc cctggttcac
     2821 cacgctctcc tccacccgcc gcatggccat gttgatgccg gcgccgatca cgatctgggc
     2881 ggcgtcgccg gtcttgccgg tcagctccac caggatgccg gccagcttcc ggtcctgcag
     2941 gtacaggtcg ttgggccact tcacccgcac cttgtcggcg cccagcttcc gcagcacctc
     3001 ggccatcacg atgccgatca ccaggctcag gccgatggcg gcggcggggc cctgctccag
     3061 ccgccagaac atgctcaggt acaggttggc gccgaagggg ctgaaccact tccggccccg
     3121 gccgccccgg ccggcctgct ggtactcggc gatgcaggcg tcgccgctct tcagctcgcc
     3181 gatccggtcc agcaggtact ggttggtgct gtcgatcacg ggcagcacgg ccacgctgcc
     3241 gccgtccagc tggcccagga tctgcttggc gttcagcagc tggatgggct cgggcaggct
     3301 gtagcccttg ccgggcacgg tgaacacgtc cacgccccag tcccgcaggg tctggatgtg
     3361 cttgttgatg gcggcccggc tcatgcccag ggtctcgccc agctgctcgc cgctgtggaa
     3421 ctcgccgttg gccagcaggg cgatcagctt caggggcacg gtgttgtcct tcattctaga
     3481 atcaaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat
     3541 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     3601 gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
     3661 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
     3721 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
     3781 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
     3841 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
     3901 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
     3961 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
     4021 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
     4081 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
     4141 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
     4201 tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata
     4261 ctgttatctg gcttttagta agccggatcc agatctttac gccccgccct gccactcatc
     4321 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     4381 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     4441 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     4501 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     4561 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     4621 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     4681 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
     4741 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     4801 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     4861 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     4921 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     4981 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
     5041 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
     5101 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     5161 tctcgttcag cttttttgta caaacttgtc taaagcttag gccgcggatc cttctccagg
     5221 cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcctac
     5281 ctcgacatac gttctctatc actgataggg agtaaactcg acatacgttc tctatcactg
     5341 atagggataa actcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt
     5401 ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata gggagtaaac
     5461 tcgacatcgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
     5521 agggagtaaa ctctagtgaa gacgaaaggg ccagacgcgg ccgctcaacg cgtgaattcg
     5581 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     5641 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     5701 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     5761 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggaactg cagcgcttac
     5821 catgtctaga ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt
     5881 cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac
     5941 cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct
     6001 ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg
     6061 gaacaacgcc aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca
     6121 tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct
     6181 gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt
     6241 tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac
     6301 acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca
     6361 gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca
     6421 gctaaagtgc gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct
     6481 cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga
     6541 ttttgacctt gacatgctcc ccgggtaact ctctgcagca gtcgacaatc aacctctgga
     6601 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6661 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6721 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6781 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6841 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6901 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6961 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7021 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7081 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7141 gacgagtcgg atctcccttt gggccgcctc cccgcctgga tcgagctcgg tacctttaag
     7201 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
     7261 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     7321 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     7381 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     7441 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     7501 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     7561 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     7621 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     7681 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     7741 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7801 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7861 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7921 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc
     7981 gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8041 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8101 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8161 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8221 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8281 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8341 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8401 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8461 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8521 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8581 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8641 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8701 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8761 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8821 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8881 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8941 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9001 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9061 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9121 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9181 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9241 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9301 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9361 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9421 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9481 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9541 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9601 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9661 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9721 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9781 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9841 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9901 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9961 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10021 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10081 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10141 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg
    10201 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    10261 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10321 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    10381 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    10441 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt
    10501 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
