LOCUS       Exported                6061 bp DNA     circular SYN 20-JUL-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     CDS             22..3834
                     /codon_start=1
                     /label=B.1.1.7_Spike_cDNA
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAISGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIF
                     GTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYHKNNKSWMESEFRVYSSANNC
                     TFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEP
                     LVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGT
                     ITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFN
                     ATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVI
                     RGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNL
                     KPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHA
                     PATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIDDTTDAVRDP
                     QTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYST
                     GSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSHRRARSVASQSIIAYTM
                     SLGAENSVAYSNNSIAIPINFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQY
                     GSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKR
                     SFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYT
                     SALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGK
                     IQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILARLDKVEAEVQ
                     IDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMS
                     FPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFY
                     EPQIITTHNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDI
                     SGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIV
                     MVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     primer_bind     complement(3897..3913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      complement(4085..4673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    complement(4790..4814)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     CDS             complement(4914..5774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4914 .. 5705 / #ccffcc
                        2: 5706 .. 5774 / #ccffcc / signal sequence
                     Cleavage site after base 5705"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5775..5879)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     5993..6009
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 gaattccagt gcggccgcac catgttcgtg ttcctggtac tccttccttt ggtgtcttcc
       61 cagtgtgtaa atcttaccac tcggacccag cttccacccg cctacaccaa cagtttcaca
      121 cgcggcgtct actatcctga caaggtgttt aggagttcag tcttgcactc aactcaagac
      181 ttgttcctcc ctttctttag caatgtgacg tggtttcatg ccatttccgg cacaaacgga
      241 acgaagcgct ttgataatcc tgtgctcccg ttcaacgatg gagtgtactt cgcgtccaca
      301 gagaagagca atatcattcg aggttggatc ttcggaacga cactcgactc aaagacgcag
      361 tcccttctca tcgtcaataa tgccacgaac gtggtcatca aagtgtgcga gtttcaattc
      421 tgtaatgatc ccttcctggg cgtctatcac aagaacaaca aatcctggat ggagtccgaa
      481 tttagagtct actccagcgc caacaactgc actttcgaat acgtatcaca gccattcttg
      541 atggaccttg aaggaaagca gggtaatttc aagaacttga gggagttcgt attcaagaat
      601 atcgacgggt actttaagat ttatagcaaa cacacaccca ttaatttggt gcgggatctt
      661 cctcagggat ttagtgctct tgagcctctc gttgacctcc ctattggcat taacatcacc
      721 cgctttcaaa ccctgttggc cctgcatcgg tcctacctga caccgggcga ctcaagttcc
      781 ggatggaccg caggtgccgc cgcatactat gtgggctacc ttcagccaag aacatttctg
      841 ctgaaatata atgagaacgg gaccattaca gatgcggtgg attgtgcact cgaccctctg
      901 tctgagacga aatgcaccct taaaagcttc acggtggaga aaggcattta tcagacttct
      961 aacttcagag ttcaacccac cgagtccatt gtgcgattcc caaatattac gaatttgtgc
     1021 ccatttggtg aggtcttcaa tgctactcga ttcgcctcag tttatgcatg gaaccgaaag
     1081 agaatttcca attgtgtggc ggactactca gtattgtata atagtgcgag ctttagcaca
     1141 ttcaaatgtt acggcgtgtc tccaacgaag ctgaacgatc tctgtttcac aaacgtttat
     1201 gcggattcct tcgtgattcg cggcgatgag gtccgacaga ttgcgcctgg gcaaacgggt
     1261 aagatcgctg attacaacta taagttgccg gacgatttca caggatgtgt catagcttgg
     1321 aatagcaata atttggacag taaggttggc ggaaactaca attatttgta caggttgttt
     1381 cgcaagtcaa atttgaaacc atttgagaga gatatatcta cggagatata tcaagccggc
     1441 tctacaccat gtaatggtgt ggagggcttt aactgctact ttccactcca gtcatatggt
     1501 ttccaaccta catatggagt agggtatcaa ccgtacagag ttgtggtctt gagtttcgaa
     1561 ttgctccacg ctccagcaac ggtatgcggt cctaagaaat ccacaaatct tgtgaagaac
     1621 aagtgcgtaa atttcaactt caatgggctg actggtaccg gcgtgctgac tgagagtaac
     1681 aagaagttct tgcctttcca acaattcggg cgggacatag acgataccac tgacgccgtc
     1741 cgcgaccctc agaccctgga gattctggac ataactcctt gttctttcgg tggcgtcagt
     1801 gttatcactc ccgggaccaa cacctccaac caagtcgcgg tcctctatca aggcgtcaac
     1861 tgtacggaag taccggtagc catccatgcg gaccaactta caccgacttg gagggtttac
     1921 tctacaggaa gcaatgtctt tcaaacacga gccgggtgtc tgatcggagc agaacacgtt
     1981 aacaacagct acgaatgtga cataccaata ggcgcaggga tttgtgcttc atatcagaca
     2041 cagaccaata gccaccggag agcgagaagc gtagcaagcc aaagcatcat cgcgtacacg
     2101 atgagcctcg gagcagagaa cagcgtcgcg tatagcaata attcaatagc tatcccaatc
     2161 aatttcacta tttcggtcac cactgagatt ctgccggtct ccatgaccaa gacatccgtc
     2221 gattgtacta tgtacatatg cggcgacagc acggagtgca gtaacttgct ccttcagtac
     2281 ggttccttct gtacgcagct taaccgggca ctgacgggta tcgcggtaga acaggacaag
     2341 aacacacagg aggtcttcgc gcaggtcaaa caaatctaca agacaccacc cataaaggac
     2401 ttcggcgggt tcaatttcag ccaaatcctg ccggaccctt ccaaacctag taagaggtca
     2461 ttcattgagg atcttctgtt taacaaagtt acgcttgcgg acgcgggatt cattaagcag
     2521 tatggtgact gccttggaga tattgccgcc agggatttga tatgtgcaca gaaatttaac
     2581 ggcctcaccg ttctgccgcc tctgctcacc gatgagatga tagcgcagta cacgagcgca
     2641 ctcctggcag gtacaattac aagcggatgg acattcggtg caggagcagc gttgcagata
     2701 ccctttgcta tgcagatggc ttatcgattt aacgggattg gcgtcacgca gaacgtcctt
     2761 tatgagaatc agaaattgat tgcaaatcag ttcaatagtg ctatcggtaa gattcaggac
     2821 agcttgagca gtaccgctag cgcactggga aagttgcagg acgtggtgaa tcagaatgca
     2881 caagcactga ataccttggt taagcaattg agtagcaatt tcggcgccat atcaagtgta
     2941 ctgaatgata tcctggctcg gttggacaag gtagaagccg aagttcagat tgaccgcttg
     3001 atcaccgggc gcctccaaag tctgcagacc tacgtcacac aacaattgat cagagcagca
     3061 gagataagag catctgctaa cctggctgcc actaagatgt ctgaatgtgt gcttgggcag
     3121 tcaaagaggg tagatttctg cggaaagggc taccacctta tgtctttccc tcagagcgct
     3181 ccgcatggtg tggtctttct ccatgtgact tatgtgcctg ctcaagagaa gaactttacg
     3241 acggcgcccg ctatatgcca tgatggtaag gcgcactttc caagggaggg agtgttcgtg
     3301 tccaacggca ctcactggtt tgtcacccaa cgaaatttct acgagcctca aattattacc
     3361 acccacaata cctttgttag cggtaactgt gacgtcgtaa ttgggattgt taataataca
     3421 gtctacgatc ctctgcagcc ggaactggac tcctttaaag aggagctgga caaatatttc
     3481 aagaaccaca catctcctga cgtagatctt ggagacataa gcggtataaa tgcaagtgtt
     3541 gttaacattc agaaagaaat agataggttg aacgaagttg cgaagaacct taacgagtca
     3601 ctgatagacc tccaagagct tgggaagtac gagcaatata tcaagtggcc ttggtatatt
     3661 tggctcgggt tcatagcagg acttatcgct atagtcatgg tgactataat gctgtgctgc
     3721 atgacaagct gctgcagctg tctcaaaggc tgttgctctt gcggctcttg ctgcaaattc
     3781 gatgaggacg attccgaacc tgtcttgaag ggtgtcaagc tccattacac ataactcgag
     3841 aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca
     3901 tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat
     3961 ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa
     4021 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4081 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4141 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4201 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4261 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4321 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4381 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4441 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4501 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4561 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4621 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4681 ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc
     4741 agcgtaaatg ggtagggggc ttcaaatcgt cctcgtgata ccaattcgga gcctgctttt
     4801 ttgtacaaac ttgttgataa tggcaattca aggatcttca cctagatcct tttaaattaa
     4861 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4921 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4981 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5041 gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5101 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5161 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5221 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5281 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5341 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5401 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5461 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5521 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5581 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5641 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5701 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5761 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5821 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5881 acatttcccc gaaaagtgcc agatacctga aacaaaaccc atcgtacggc caaggaagtc
     5941 tccaataact gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg
     6001 acggccagtc atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc
     6061 t
//
