LOCUS       Exported                6064 bp DNA     circular SYN 20-JUL-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     CDS             22..3837
                     /codon_start=1
                     /label=B.1.617.2_Spike_cDNA
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNLRTRTQLPPAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESGVYSSANN
                     CTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALE
                     PLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENG
                     TITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVF
                     NATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFV
                     IRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYRYRLFRKSN
                     LKPFERDISTEIYQAGSKPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLH
                     APATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRD
                     PQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYS
                     TGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSRRRARSVASQSIIAYT
                     MSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQ
                     YGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSK
                     RSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQY
                     TSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIG
                     KIQDSLSSTASALGKLQNVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEV
                     QIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLM
                     SFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNF
                     YEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGD
                     ISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAI
                     VMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     primer_bind     complement(3900..3916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      complement(4088..4676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    complement(4793..4817)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     CDS             complement(4917..5777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4917 .. 5708 / #ccffcc
                        2: 5709 .. 5777 / #ccffcc / signal sequence
                     Cleavage site after base 5708"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5778..5882)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     5996..6012
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 gaattccagt gcggccgcac catgttcgtg ttcctggtac tccttccttt ggtgtcttcc
       61 cagtgtgtaa atcttcgcac tcggacccag cttccacccg cctacaccaa cagtttcaca
      121 cgcggcgtct actatcctga caaggtgttt aggagttcag tcttgcactc aactcaagac
      181 ttgttcctcc ctttctttag caatgtgacg tggtttcatg ccattcatgt ctccggcaca
      241 aacggaacga agcgctttga taatcctgtg ctcccgttca acgatggagt gtacttcgcg
      301 tccacagaga agagcaatat cattcgaggt tggatcttcg gaacgacact cgactcaaag
      361 acgcagtccc ttctcatcgt caataatgcc acgaacgtgg tcatcaaagt gtgcgagttt
      421 caattctgta atgatccctt cctgggcgtc tattatcaca agaacaacaa atcctggatg
      481 gagtccggag tctactccag cgccaacaac tgcactttcg aatacgtatc acagccattc
      541 ttgatggacc ttgaaggaaa gcagggtaat ttcaagaact tgagggagtt cgtattcaag
      601 aatatcgacg ggtactttaa gatttatagc aaacacacac ccattaattt ggtgcgggat
      661 cttcctcagg gatttagtgc tcttgagcct ctcgttgacc tccctattgg cattaacatc
      721 acccgctttc aaaccctgtt ggccctgcat cggtcctacc tgacaccggg cgactcaagt
      781 tccggatgga ccgcaggtgc cgccgcatac tatgtgggct accttcagcc aagaacattt
      841 ctgctgaaat ataatgagaa cgggaccatt acagatgcgg tggattgtgc actcgaccct
      901 ctgtctgaga cgaaatgcac ccttaaaagc ttcacggtgg agaaaggcat ttatcagact
      961 tctaacttca gagttcaacc caccgagtcc attgtgcgat tcccaaatat tacgaatttg
     1021 tgcccatttg gtgaggtctt caatgctact cgattcgcct cagtttatgc atggaaccga
     1081 aagagaattt ccaattgtgt ggcggactac tcagtattgt ataatagtgc gagctttagc
     1141 acattcaaat gttacggcgt gtctccaacg aagctgaacg atctctgttt cacaaacgtt
     1201 tatgcggatt ccttcgtgat tcgcggcgat gaggtccgac agattgcgcc tgggcaaacg
     1261 ggtaagatcg ctgattacaa ctataagttg ccggacgatt tcacaggatg tgtcatagct
     1321 tggaatagca ataatttgga cagtaaggtt ggcggaaact acaattatcg atacaggttg
     1381 tttcgcaagt caaatttgaa accatttgag agagatatat ctacggagat atatcaagcc
     1441 ggctctaagc catgtaatgg tgtggagggc tttaactgct actttccact ccagtcatat
     1501 ggtttccaac ctacaaatgg agtagggtat caaccgtaca gagttgtggt cttgagtttc
     1561 gaattgctcc acgctccagc aacggtatgc ggtcctaaga aatccacaaa tcttgtgaag
     1621 aacaagtgcg taaatttcaa cttcaatggg ctgactggta ccggcgtgct gactgagagt
     1681 aacaagaagt tcttgccttt ccaacaattc gggcgggaca tagctgatac cactgacgcc
     1741 gtccgcgacc ctcagaccct ggagattctg gacataactc cttgttcttt cggtggcgtc
     1801 agtgttatca ctcccgggac caacacctcc aaccaagtcg cggtcctcta tcaaggcgtc
     1861 aactgtacgg aagtaccggt agccatccat gcggaccaac ttacaccgac ttggagggtt
     1921 tactctacag gaagcaatgt ctttcaaaca cgagccgggt gtctgatcgg agcagaacac
     1981 gttaacaaca gctacgaatg tgacatacca ataggcgcag ggatttgtgc ttcatatcag
     2041 acacagacca atagcagacg gagagcgaga agcgtagcaa gccaaagcat catcgcgtac
     2101 acgatgagcc tcggagcaga gaacagcgtc gcgtatagca ataattcaat agctatccca
     2161 acaaatttca ctatttcggt caccactgag attctgccgg tctccatgac caagacatcc
     2221 gtcgattgta ctatgtacat atgcggcgac agcacggagt gcagtaactt gctccttcag
     2281 tacggttcct tctgtacgca gcttaaccgg gcactgacgg gtatcgcggt agaacaggac
     2341 aagaacacac aggaggtctt cgcgcaggtc aaacaaatct acaagacacc acccataaag
     2401 gacttcggcg ggttcaattt cagccaaatc ctgccggacc cttccaaacc tagtaagagg
     2461 tcattcattg aggatcttct gtttaacaaa gttacgcttg cggacgcggg attcattaag
     2521 cagtatggtg actgccttgg agatattgcc gccagggatt tgatatgtgc acagaaattt
     2581 aacggcctca ccgttctgcc gcctctgctc accgatgaga tgatagcgca gtacacgagc
     2641 gcactcctgg caggtacaat tacaagcgga tggacattcg gtgcaggagc agcgttgcag
     2701 ataccctttg ctatgcagat ggcttatcga tttaacggga ttggcgtcac gcagaacgtc
     2761 ctttatgaga atcagaaatt gattgcaaat cagttcaata gtgctatcgg taagattcag
     2821 gacagcttga gcagtaccgc tagcgcactg ggaaagttgc agaacgtggt gaatcagaat
     2881 gcacaagcac tgaatacctt ggttaagcaa ttgagtagca atttcggcgc catatcaagt
     2941 gtactgaatg atatcctgtc acggttggac aaggtagaag ccgaagttca gattgaccgc
     3001 ttgatcaccg ggcgcctcca aagtctgcag acctacgtca cacaacaatt gatcagagca
     3061 gcagagataa gagcatctgc taacctggct gccactaaga tgtctgaatg tgtgcttggg
     3121 cagtcaaaga gggtagattt ctgcggaaag ggctaccacc ttatgtcttt ccctcagagc
     3181 gctccgcatg gtgtggtctt tctccatgtg acttatgtgc ctgctcaaga gaagaacttt
     3241 acgacggcgc ccgctatatg ccatgatggt aaggcgcact ttccaaggga gggagtgttc
     3301 gtgtccaacg gcactcactg gtttgtcacc caacgaaatt tctacgagcc tcaaattatt
     3361 accaccgaca atacctttgt tagcggtaac tgtgacgtcg taattgggat tgttaataat
     3421 acagtctacg atcctctgca gccggaactg gactccttta aagaggagct ggacaaatat
     3481 ttcaagaacc acacatctcc tgacgtagat cttggagaca taagcggtat aaatgcaagt
     3541 gttgttaaca ttcagaaaga aatagatagg ttgaacgaag ttgcgaagaa ccttaacgag
     3601 tcactgatag acctccaaga gcttgggaag tacgagcaat atatcaagtg gccttggtat
     3661 atttggctcg ggttcatagc aggacttatc gctatagtca tggtgactat aatgctgtgc
     3721 tgcatgacaa gctgctgcag ctgtctcaaa ggctgttgct cttgcggctc ttgctgcaaa
     3781 ttcgatgagg acgattccga acctgtcttg aagggtgtca agctccatta cacataactc
     3841 gagaggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca tgtgtcatgg
     3901 tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc
     3961 tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg
     4021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4081 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4501 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4681 atcctttgat cttttctacg gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa
     4741 gtcagcgtaa atgggtaggg ggcttcaaat cgtcctcgtg ataccaattc ggagcctgct
     4801 tttttgtaca aacttgttga taatggcaat tcaaggatct tcacctagat ccttttaaat
     4861 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4921 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4981 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5041 gctgcaatga taccgcgaga gccacgctca ccggctccag atttatcagc aataaaccag
     5101 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5161 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5221 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5281 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5341 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5401 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5461 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5521 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5581 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5641 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5701 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5761 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5821 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5881 cgcacatttc cccgaaaagt gccagatacc tgaaacaaaa cccatcgtac ggccaaggaa
     5941 gtctccaata actgtgatcc accacaagcg ccagggtttt cccagtcacg acgttgtaaa
     6001 acgacggcca gtcatgcata atccgcacgc atctggaata aggaagtgcc attccgcctg
     6061 acct
//
