LOCUS       Exported                6070 bp DNA     circular SYN 20-JUL-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     CDS             22..3843
                     /codon_start=1
                     /label=P.1_Spike_cDNA
                     /note="color: #993366"
                     /translation="MFVFLVLLPLVSSQCVNFTNRTQLPSAYTNSFTRGVYYPDKVFRS
                     SVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGW
                     IFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNYPFLGVYYHKNNKSWMESEFRVYSSA
                     NNCTFEYVSQPFLMDLEGKQGNFKNLSEFVFKNIDGYFKIYSKHTPINLVRDLPQGFSA
                     LEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNE
                     NGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGE
                     VFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADS
                     FVIRGDEVRQIAPGQTGTIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRK
                     SNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFEL
                     LHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAV
                     RDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRV
                     YSTGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIA
                     YTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLL
                     LQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKP
                     SKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIA
                     QYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSA
                     IGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEA
                     EVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAAIKMSECVLGQSKRVDFCGKGYH
                     LMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQR
                     NFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDL
                     GDISGINASFVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLI
                     AIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT"
     primer_bind     complement(3906..3922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      complement(4094..4682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    complement(4799..4823)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     CDS             complement(4923..5783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4923 .. 5714 / #ccffcc
                        2: 5715 .. 5783 / #ccffcc / signal sequence
                     Cleavage site after base 5714"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5784..5888)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     6002..6018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 gaattccagt gcggccgcac catgttcgtg ttcctggtac tccttccttt ggtgtcttcc
       61 cagtgtgtaa attttaccaa tcggacccag cttccatccg cctacaccaa cagtttcaca
      121 cgcggcgtct actatcctga caaggtgttt aggagttcag tcttgcactc aactcaagac
      181 ttgttcctcc ctttctttag caatgtgacg tggtttcatg ccattcatgt ctccggcaca
      241 aacggaacga agcgctttga taatcctgtg ctcccgttca acgatggagt gtacttcgcg
      301 tccacagaga agagcaatat cattcgaggt tggatcttcg gaacgacact cgactcaaag
      361 acgcagtccc ttctcatcgt caataatgcc acgaacgtgg tcatcaaagt gtgcgagttt
      421 caattctgta attatccctt cctgggcgtc tattatcaca agaacaacaa atcctggatg
      481 gagtccgaat ttagagtcta ctccagcgcc aacaactgca ctttcgaata cgtatcacag
      541 ccattcttga tggaccttga aggaaagcag ggtaatttca agaacttgag tgagttcgta
      601 ttcaagaata tcgacgggta ctttaagatt tatagcaaac acacacccat taatttggtg
      661 cgggatcttc ctcagggatt tagtgctctt gagcctctcg ttgacctccc tattggcatt
      721 aacatcaccc gctttcaaac cctgttggcc ctgcatcggt cctacctgac accgggcgac
      781 tcaagttccg gatggaccgc aggtgccgcc gcatactatg tgggctacct tcagccaaga
      841 acatttctgc tgaaatataa tgagaacggg accattacag atgcggtgga ttgtgcactc
      901 gaccctctgt ctgagacgaa atgcaccctt aaaagcttca cggtggagaa aggcatttat
      961 cagacttcta acttcagagt tcaacccacc gagtccattg tgcgattccc aaatattacg
     1021 aatttgtgcc catttggtga ggtcttcaat gctactcgat tcgcctcagt ttatgcatgg
     1081 aaccgaaaga gaatttccaa ttgtgtggcg gactactcag tattgtataa tagtgccagc
     1141 tttagcacat tcaaatgtta cggcgtgtct ccaacgaagc tgaacgatct ctgtttcaca
     1201 aacgtttatg cggattcctt cgtgattcgc ggcgatgagg tccgacagat tgcgcctggg
     1261 caaacgggta cgatcgctga ttacaactat aagttgccgg acgatttcac aggatgtgtc
     1321 atagcttgga atagcaataa tttggacagt aaggttggcg gaaactacaa ttatttgtac
     1381 aggttgtttc gcaagtcaaa tttgaaacca tttgagagag atatatctac ggagatatat
     1441 caagccggct ctacaccatg taatggtgtg aagggcttta actgctactt tccactccag
     1501 tcatatggtt tccaacctac atatggagta gggtatcaac cgtacagagt tgtggtcttg
     1561 agtttcgaat tgctccacgc tccagcaacg gtatgcggtc ctaagaaatc cacaaatctt
     1621 gtgaagaaca agtgcgtaaa tttcaacttc aatgggctga ctggtaccgg cgtgctgact
     1681 gagagtaaca agaagttctt gcctttccaa caattcgggc gggacatagc tgataccact
     1741 gacgccgtcc gcgaccctca gaccctggag attctggaca taactccttg ttctttcggt
     1801 ggcgtcagtg ttatcactcc cgggaccaac acctccaacc aagtcgcggt cctctatcaa
     1861 ggcgtcaact gtacggaagt accggtagcc atccatgcgg accaacttac accgacttgg
     1921 agggtttact ctacaggaag caatgtcttt caaacacgag ccgggtgtct gatcggagca
     1981 gaatacgtta acaacagcta cgaatgtgac ataccaatag gcgcagggat ttgtgcttca
     2041 tatcagacac agaccaatag cccgcggaga gcgagaagcg tagcaagcca aagcatcatc
     2101 gcgtacacga tgagcctcgg agcagagaac agcgtcgcgt atagcaataa ttcaatagct
     2161 atcccaacaa atttcactat ttcggtcacc actgagattc tgccggtctc catgaccaag
     2221 acatccgtcg attgtactat gtacatatgc ggcgacagca cggagtgcag taacttgctc
     2281 cttcagtacg gttccttctg tacgcagctt aaccgggcac tgacgggtat cgcggtagaa
     2341 caggacaaga acacacagga ggtcttcgcg caggtcaaac aaatctacaa gacaccaccc
     2401 ataaaggact tcggcgggtt caatttcagc caaatcctgc cggacccttc caaacctagt
     2461 aagaggtcat tcattgagga tcttctgttt aacaaagtta cgcttgcgga cgcgggattc
     2521 attaagcagt atggtgactg ccttggagat attgccgcca gggatttgat atgtgcacag
     2581 aaatttaacg gcctcaccgt tctgccgcct ctgctcaccg atgagatgat agcgcagtac
     2641 acgagcgcac tcctggcagg tacaattaca agcggatgga cattcggtgc aggagcagcg
     2701 ttgcagatac cctttgctat gcagatggct tatcgattta acgggattgg cgtcacgcag
     2761 aacgtccttt atgagaatca gaaattgatt gcaaatcagt tcaatagtgc tatcggtaag
     2821 attcaggaca gcttgagcag taccgctagc gcactgggaa agttgcagga cgtggtgaat
     2881 cagaatgcac aagcactgaa taccttggtt aagcaattga gtagcaattt cggcgccata
     2941 tcaagtgtac tgaatgatat cctgtcacgg ttggacaagg tagaagccga agttcagatt
     3001 gaccgcttga tcaccgggcg cctccaaagt ctgcagacct acgtcacaca acaattgatc
     3061 agagcagcag agataagagc atctgctaac ctggctgcca ttaagatgtc tgaatgtgtg
     3121 cttgggcagt caaagagggt agatttctgc ggaaagggct accaccttat gtctttccct
     3181 cagagcgctc cgcatggtgt ggtctttctc catgtgactt atgtgcctgc tcaagagaag
     3241 aactttacga cggcgcccgc tatatgccat gatggtaagg cgcactttcc aagggaggga
     3301 gtgttcgtgt ccaacggcac tcactggttt gtcacccaac gaaatttcta cgagcctcaa
     3361 attattacca ccgacaatac ctttgttagc ggtaactgtg acgtcgtaat tgggattgtt
     3421 aataatacag tctacgatcc tctgcagccg gaactggact cctttaaaga ggagctggac
     3481 aaatatttca agaaccacac atctcctgac gtagatcttg gagacataag cggtataaat
     3541 gcaagttttg ttaacattca gaaagaaata gataggttga acgaagttgc gaagaacctt
     3601 aacgagtcac tgatagacct ccaagagctt gggaagtacg agcaatatat caagtggcct
     3661 tggtatattt ggctcgggtt catagcagga cttatcgcta tagtcatggt gactataatg
     3721 ctgtgctgca tgacaagctg ctgcagctgt ctcaaaggct gttgctcttg cggctcttgc
     3781 tgcaaattcg atgaggacga ttccgaacct gtcttgaagg gtgtcaagct ccattacaca
     3841 taactcgaga ggctaggtgg aggctcagtg atgataagtc tgcgatggtg gatgcatgtg
     3901 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca caatcctatt
     3961 ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt atggcatatg
     4021 tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4501 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4681 aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa gccgccgtcc
     4741 cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc ctcgtgatac caattcggag
     4801 cctgcttttt tgtacaaact tgttgataat ggcaattcaa ggatcttcac ctagatcctt
     4861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4921 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4981 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5041 cccagtgctg caatgatacc gcgagagcca cgctcaccgg ctccagattt atcagcaata
     5101 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5161 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5221 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5281 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5341 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5401 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5461 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5521 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5581 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5641 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5701 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5761 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5821 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5881 gttccgcgca catttccccg aaaagtgcca gatacctgaa acaaaaccca tcgtacggcc
     5941 aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca gtcacgacgt
     6001 tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc
     6061 cgcctgacct
//
